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Related Experiment Video

Updated: Feb 17, 2026

High-resolution Fiber-optic Microendoscopy for in situ Cellular Imaging

High-resolution Fiber-optic Microendoscopy for in situ Cellular Imaging

Published on: January 11, 2011

Multimode fibre based imaging for optically cleared samples.

Ivan Gusachenko¹, Jonathan Nylk¹, Javier A Tello²

¹SUPA, School of Physics and Astronomy, University of St. Andrews, Fife, KY16 9SS, UK.

Biomedical Optics Express

|December 1, 2017

Summary

This summary is machine-generated.

This study introduces a corrosion-resistant multimode fiber for optical clearing in neuroscience. This versatile approach enables imaging through cleared tissues and in harsh environments, overcoming limitations of traditional methods.

Keywords:

(070.6120) Spatial light modulators (110.2350) Fiber optics imaging (110.7348) Wavefront encoding (170.3660) Light propagation in tissues (180.2520) Fluorescence microscopy (180.5655) Raman microscopy

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Area of Science:

Neuroscience
Optical Imaging
Biophotonics

Background:

Optical clearing techniques are vital for deep tissue imaging in neuroscience.
Corrosive clearing solutions pose challenges for conventional microscopy objectives.
Existing methods often require specialized optics or careful handling of optical components.

Purpose of the Study:

To present a novel, corrosion-resistant imaging approach for optically cleared tissues.
To demonstrate the utility of corrected multimode fibers (MMF) as an alternative to complex objectives.
To showcase the versatility of MMF for various imaging modalities and challenging environments.

Main Methods:

Utilized a corrected multimode fiber (MMF) compatible with clearing media.
Performed fluorescence imaging of fluorescent beads and stained neuroblastoma cells.
Conducted imaging experiments in an extreme oxidative environment.
Applied Raman imaging to polystyrene beads in clearing media.

Main Results:

Demonstrated successful fluorescence imaging through optically cleared mouse brain tissue using MMF.
Showcased MMF's resilience in an extreme oxidative environment, highlighting its versatility.
Confirmed the applicability of MMF for vibrational spectroscopy (Raman imaging) of cleared samples.

Conclusions:

Corrected multimode fibers offer a robust, flexible, and corrosion-resistant solution for optical clearing applications.
This MMF approach circumvents limitations associated with refractive index matching and working distance.
The method extends the capabilities of optical imaging and spectroscopy in cleared biological tissues.