A chemoselective cleavable fluorescence turn-ON linker for proteomic studies

Area of Science:

• Biochemistry
• Chemical Biology
• Proteomics

Background:

• Chemoproteomics requires advanced tools for in situ analysis.
• Developing novel linkers is crucial for expanding proteomic capabilities.

Purpose of the Study:

• To develop a trifunctional, cleavable, fluorescence turn-ON linker for chemoproteomic applications.
• To demonstrate the linker's utility in proteome profiling and target identification.

Main Methods:

• Synthesis of a novel trifunctional linker featuring an azo bond.
• Utilizing the linker's fluorescence turn-ON property upon azo bond cleavage.
• Application of the linker for in situ proteome profiling and target identification.

Main Results:

• The developed linker exhibits fluorescence exclusively after azo bond cleavage.
• Successful application of the linker for in situ proteome profiling.
• Demonstrated utility in identifying targets within newly synthesized proteomes.

Conclusions:

• The trifunctional cleavable linker is a valuable tool for chemoproteomics.
• This linker enables sensitive and specific proteome profiling and target identification.
• The linker opens new avenues for studying complex proteomes.