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Updated: Feb 17, 2026

Discrimination of Seven Immune Cell Subsets by Two-fluorochrome Flow Cytometry
Published on: March 5, 2019
Flow cytometric discrimination of seven lineage markers by using two fluorochromes
Francesco Boin1, Maria Letizia Giardino Torchia2, Ivan Borrello3
1Division of Rheumatology, Johns Hopkins University School of Medicine, Baltimore, Maryland, United States of America.
Abstract:
Flow cytometry is the primary immunological technique used to analyze multiple parameters on complex cell populations. We present a staining method that identifies major human mononuclear lymphoid and myeloid populations (CD4+ and CD8+ T cells, γδ T cells, B cells, NK cells and monocytes), using only two fluorochromes and a minimal number of cells. Our approach increases the number of markers recordable on most flow cytometers allowing for a deeper and more comprehensive immunophenotyping.

