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Novel controlled ionic gelation strategy for chitosan nanoparticles preparation using TPP-β-CD inclusion complex
Anjali Pant1, Jeetendra Singh Negi2
1Department of Pharmaceutical Sciences, S Bhagwan Singh PG Institute of Bio-medical Sciences and research, Balawala, Dehradun 248161, Uttarakhand, India.
Summary
This study introduces a new method using a sodium tripolyphosphate (TPP) and beta cyclodextrin (β-CD) complex to create smaller, more uniform chitosan nanoparticles, overcoming aggregation issues. This controlled ionic gelation improves nanoparticle characteristics for potential applications.
Area of Science:
- Materials Science
- Nanotechnology
- Polymer Chemistry
Background:
- Chitosan nanoparticles are widely used but conventional ionic gelation often leads to particle aggregation.
- Developing controlled methods to improve nanoparticle size and uniformity is crucial for enhanced efficacy.
Purpose of the Study:
- To develop a novel controlled ionic gelation strategy for chitosan nanoparticle preparation.
- To investigate the inclusion complexation of sodium tripolyphosphate (TPP) with beta cyclodextrin (β-CD).
- To utilize the TPP-β-CD inclusion complex for preparing chitosan nanoparticles with improved characteristics.
Main Methods:
- Characterization of the TPP-β-CD inclusion complex using FT-IR, XRD, and DSC.
- Molecular docking studies to investigate complexation behavior.
- Preparation of chitosan nanoparticles using the TPP-β-CD inclusion complex.
- Analysis of nanoparticle size, polydispersity index (PDI), and zeta potential.
- Surface morphology observation using transmission electron microscopy (TEM).
Main Results:
- The TPP molecule successfully formed an inclusion complex with β-CD.
- Chitosan nanoparticles prepared using the TPP-β-CD complex exhibited a smaller particle size (104.2nm ± 0.608).
- The nanoparticles showed a good PDI value (0.346 ± 0.016) and acceptable zeta potential (+27.33mV ± 0.416).
- TEM analysis confirmed the formation of smaller and more uniform nanoparticles compared to conventional methods.
Conclusions:
- The TPP-β-CD inclusion complex provides an effective strategy for controlled ionic gelation.
- This novel approach yields chitosan nanoparticles with superior size control and uniformity.
- The developed method offers an advantage over conventional TPP-based chitosan nanoparticle preparation, reducing aggregation.
