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Updated: Feb 17, 2026
Purification and Expansion of Mouse Invariant Natural Killer T Cells for in vitro and in vivo Studies
Published on: February 15, 2021
Improved Detection of Cytokines Produced by Invariant NKT Cells
Duygu Sag1,2, Müge Özkan3, Mitchell Kronenberg4,5
1Izmir Biomedicine and Genome Center (IBG), 35340, Balcova, Izmir, Turkey.
Abstract:
Invariant Natural killer T (iNKT) cells rapidly produce copious amounts of multiple cytokines after in vivo activation, allowing for the direct detection of a number of cytokines directly ex vivo. However, for some cytokines this approach is suboptimal. Here, we report technical variations that allow the improved detection of IL-4, IL-10, IL-13 and IL-17A ex vivo. Furthermore, we describe an alternative approach for stimulation of iNKT cells in vitro that allows a significantly improved detection of cytokines produced by iNKT cells. Together, these protocols allow the detection of iNKT cell cytokines ex vivo and in vitro with increased sensitivity.
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