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Related Concept Videos

Immunoprecipitation01:20

Immunoprecipitation

7.6K
Immunoprecipitation, or IP, is a widely used technique that employs protein-antibody interactions to isolate proteins or protein complexes in their native state for studying protein-protein interactions, quaternary structures, or supramolecular complexes. Various modifications of the technique, including chromatin IP, cross-linking IP, and fluorescence IP, are commonly used.
Chromatin Immunoprecipitation
Chromatin immunoprecipitation, also known as ChIP, is used to study protein-DNA or...
7.6K
Chromatin Immunoprecipitation- ChIP02:36

Chromatin Immunoprecipitation- ChIP

12.6K
Chromatin immunoprecipitation, or ChIP, is an antibody-based technique used to identify sites on DNA that bind to transcription factors of interest or histone proteins. It also helps determine the type of histone modifications such as acetylation, phosphorylation, or methylation.
Types of ChIP
ChIP can be divided into two types - X-ChIP and N-ChIP. X-ChIP involves in vivo cross-linking of histones and regulatory proteins to DNA, fragmenting the DNA by sonication, and isolating the protein-DNA...
12.6K
Precipitation and Co-precipitation01:17

Precipitation and Co-precipitation

4.4K
Precipitation and coprecipitation methods can be used to separate a mixture of ions in a solution. In qualitative inorganic analysis, ions that form sparingly soluble precipitates with the same reagent are separated based on the differences in solubility products. For example, consider the separation of Cu(II) and Fe(II) ions by precipitation as insoluble sulfides. First, copper(II) sulfide is precipitated by the addition of acidic H2S, where the dissociation of H2S is suppressed. Adding H2S...
4.4K
Immunogold Electron Microscopy01:20

Immunogold Electron Microscopy

5.6K
Immunoelectron microscopy utilizes immunogold labeling of endogenous proteins with specific antibodies to detect and localize these proteins in cells and tissues. The procedure provides insights into the distribution and quantification of protein under different stimulation conditions offering clues about their functions. Conjugating highly electron-dense gold particles with primary or secondary antibodies allow antigen detection on and within cells, with high resolution and specificity.
5.6K
Types of Coprecipitation01:10

Types of Coprecipitation

6.8K
Coprecipitation is the contamination of a precipitate by otherwise soluble species and occurs via different processes. In colloidal precipitates, coprecipitation occurs via surface adsorption. For instance, barium sulfate has a primary layer of adsorbed barium ions and a secondary layer of nitrate counterions. This results in contamination of the precipitate by barium nitrate.
Sometimes, ions in a crystal lattice can undergo isomorphous replacement by inclusions of similar charge and size. For...
6.8K

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Immunoprecipitation.

James DeCaprio, Thomas O Kohl

    Cold Spring Harbor Protocols
    |December 3, 2017
    PubMed
    Summary
    This summary is machine-generated.

    This protocol outlines protein enrichment and purification using immunoprecipitation. The purified protein targets can be analyzed via assays like ELISA, western blotting, or mass spectrometry.

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    Area of Science:

    • Biochemistry
    • Molecular Biology
    • Immunology

    Background:

    • Protein purification is essential for biochemical and molecular biology research.
    • Complex biological samples require methods for isolating specific target proteins.

    Purpose of the Study:

    • To provide a detailed protocol for immunoprecipitation.
    • To enable enrichment and purification of specific proteins from cell lysates.

    Main Methods:

    • Utilizes antibodies bound to a solid matrix for specific antigen capture.
    • Describes sequential steps for protein isolation and purification.
    • Includes various elution techniques for releasing purified proteins.

    Main Results:

    • Successful enrichment and purification of target proteins from complex cell lysates.
    • Demonstrates the versatility of the protocol for different protein targets.

    Conclusions:

    • The described immunoprecipitation protocol is effective for isolating specific proteins.
    • Purified proteins are suitable for downstream analysis using techniques such as ELISA, western blotting, and mass spectrometry.