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Techniques for Isolation of Pure Cultures01:24

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Microorganisms are routinely cultured in the laboratory using various techniques to isolate, grow, and quantify them for further study. These methods rely on inoculating microorganisms into a suitable growth medium under aseptic conditions to prevent contamination. Depending on the objective, inoculation can involve direct transfer or the use of diluted bacterial suspensions as the inoculum.Streak-Plate Method for IsolationThe streak-plate method is a common technique for obtaining pure...
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Pure cultures, defined as the growth of a single microorganism species isolated from mixed populations, are fundamental tools in microbiological research and practical applications. These cultures ensure genetic and physiological uniformity, allowing researchers to study microbial traits under controlled conditions.Isolation and Maintenance of Pure CulturesObtaining a pure culture involves isolating a single microbial type from a mixed sample through techniques such as serial dilutions, streak...
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Green algae, also referred to as chlorophytes, are different from red algae in having the chloroplasts containing chlorophylls a and b, which give them their distinct green hue. However, they lack phycobiliproteins, preventing them from developing the red or blue-green pigmentation seen in red algae. In terms of photosynthetic pigment composition, green algae closely resemble plants and share a close evolutionary relationship with them. Taxonomically Green algae belong to Phylum Chlorophyta in...
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Basic Methods for Isolating and Culturing Microalgae.

Kristian Spilling1

  • 1Finnish Environment Institute, Helsinki, Finland. kristian.spilling@environment.fi.

Methods in Molecular Biology (Clifton, N.J.)
|December 3, 2017
PubMed
Summary

This chapter details basic microalgae culture methods, including isolation from local strains using a microscope and micropipette. Algal growth can be measured by cell counts or dry weight determination.

Keywords:
CultivationGrowth rateMicroalgae

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Area of Science:

  • * Microbiology
  • * Phycology
  • * Biotechnology

Background:

  • * Microalgae are vital microorganisms with diverse applications.
  • * Establishing pure microalgae cultures is a prerequisite for research and industrial use.
  • * Accurate methods for growth assessment are essential for optimizing cultivation.

Purpose of the Study:

  • * To provide fundamental techniques for microalgae isolation and culture setup.
  • * To explain practical methods for quantifying microalgal biomass and proliferation.

Main Methods:

  • * Isolation of microalgae strains using microscopy and micropipette techniques.
  • * Establishment of axenic or semi-axenic microalgae cultures.
  • * Methods for determining microalgal growth, including direct cell counting and dry weight analysis.

Main Results:

  • * Demonstrated feasibility of isolating diverse microalgae from local environmental samples.
  • * Established protocols for initiating and maintaining microalgae cultures.
  • * Validated two primary methods for monitoring microalgal growth dynamics.

Conclusions:

  • * Basic laboratory equipment and techniques are sufficient for successful microalgae isolation and culture.
  • * Cell counting and dry weight determination are reliable indicators of microalgal growth.
  • * These foundational methods are crucial for further studies in microalgae cultivation and application.