Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Low-Grade Inflammation Aggravates Rotenone Neurotoxicity and Disrupts Circadian Clock Gene Expression in Rats.

Neurotoxicity research·2018
Same author

Change in the intestinal bacterial community structure associated with environmental microorganisms during the growth of Eriocheir sinensis.

MicrobiologyOpen·2018
Same author

Transcriptomic analyses identify albino-associated genes of a novel albino tea germplasm 'Huabai 1'.

Horticulture research·2018
Same author

New phenolic halogenated disinfection byproducts in simulated chlorinated drinking water: Identification, decomposition, and control by ozone-activated carbon treatment.

Water research·2018
Same author

Oligonucleotide Aptamer-Mediated Precision Therapy of Hematological Malignancies.

Molecular therapy. Nucleic acids·2018
Same author

Effects of consecutive monoculture of sweet potato on soil bacterial community as determined by pyrosequencing.

Journal of basic microbiology·2018

Related Experiment Video

Updated: Feb 17, 2026

The Development of Lyophilized Loop-mediated Isothermal Amplification Reagents for the Detection of Coxiella burnetii
07:27

The Development of Lyophilized Loop-mediated Isothermal Amplification Reagents for the Detection of Coxiella burnetii

Published on: April 18, 2016

10.9K

Rapid visual detection of binary toxin producing Clostridium difficile by loop-mediated isothermal amplification.

Lan Yu1, Huan Li2, Xiangna Zhao2

  • 1Department of Gastroenterology, Navy General Hospital, Beijing 100048, P.R. China.

Experimental and Therapeutic Medicine
|December 5, 2017
PubMed
Summary

Loop-mediated isothermal amplification (LAMP) offers a rapid and visual method for detecting Clostridium difficile transferase (CDT). This diagnostic tool aids in identifying severe C. difficile infections at the point of care.

Keywords:
Clostridium difficilebinary toxin C. difficile transferasecalceinloop-mediated isothermal amplificationturbidity

More Related Videos

Author Spotlight: Advancing Rapid Detection of Respiratory Pathogens Using Microfluidic Chip
06:11

Author Spotlight: Advancing Rapid Detection of Respiratory Pathogens Using Microfluidic Chip

Published on: March 29, 2024

2.4K
Visual Detection of Multiple Nucleic Acids in a Capillary Array
08:56

Visual Detection of Multiple Nucleic Acids in a Capillary Array

Published on: November 15, 2017

7.8K

Related Experiment Videos

Last Updated: Feb 17, 2026

The Development of Lyophilized Loop-mediated Isothermal Amplification Reagents for the Detection of Coxiella burnetii
07:27

The Development of Lyophilized Loop-mediated Isothermal Amplification Reagents for the Detection of Coxiella burnetii

Published on: April 18, 2016

10.9K
Author Spotlight: Advancing Rapid Detection of Respiratory Pathogens Using Microfluidic Chip
06:11

Author Spotlight: Advancing Rapid Detection of Respiratory Pathogens Using Microfluidic Chip

Published on: March 29, 2024

2.4K
Visual Detection of Multiple Nucleic Acids in a Capillary Array
08:56

Visual Detection of Multiple Nucleic Acids in a Capillary Array

Published on: November 15, 2017

7.8K

Area of Science:

  • Microbiology
  • Molecular Diagnostics
  • Infectious Diseases

Background:

  • Clostridium difficile transferase (CDT) is a binary toxin found in C. difficile strains.
  • CDT-producing C. difficile infections are linked to increased disease severity and higher fatality rates.
  • Accurate and rapid identification of CDT-producing strains is crucial for effective patient management.

Purpose of the Study:

  • To develop and validate a loop-mediated isothermal amplification (LAMP) assay for detecting CDT-producing C. difficile.
  • To assess the feasibility of visual detection for point-of-care application.
  • To evaluate the sensitivity and specificity of the developed LAMP assay.

Main Methods:

  • Loop-mediated isothermal amplification (LAMP) was employed to detect CDT-producing C. difficile.
  • Two detection methods were utilized: real-time turbidity monitoring and visual detection with calcein.
  • Primer specificity was confirmed using 25 non-CDT-producing bacterial strains.

Main Results:

  • The LAMP assay successfully amplified and visually detected target DNA within 60 minutes at 60°C.
  • The assay demonstrated a sensitivity 10-fold greater than traditional polymerase chain reaction (PCR) analysis.
  • High specificity was confirmed, with no amplification observed in strains lacking CDT.

Conclusions:

  • A visual LAMP method for detecting CDT-producing C. difficile has been successfully established.
  • This method is rapid, reliable, and cost-effective.
  • The visual LAMP assay holds significant potential for point-of-care diagnostics of C. difficile infections.