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Related Concept Videos

Instrument Calibration01:12

Instrument Calibration

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Instrument calibration is essential for ensuring that instruments produce accurate and consistent results. It is vital in manufacturing, healthcare, testing laboratories, and scientific research. Calibration processes are specific to each instrument and help enhance data accuracy. Each instrument has a unique calibration process tailored to its design and function to improve data accuracy.
Analytical Balance Calibration
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Testing a Claim about Standard Deviation01:19

Testing a Claim about Standard Deviation

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A complete procedure to test a claim about population standard deviation or population variance is explained here.
The hypothesis testing for the claim of population standard deviation (or variance) requires the data and samples to be random and unbiased. The population distribution also must be normal. There is no specific requirement on the sample size as the estimation is based on the chi-square distribution.
As a first step, the hypothesis (null and alternative) concerning the claim about...
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Glassware Calibration01:11

Glassware Calibration

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Accurate calibration of glassware, such as volumetric flasks, pipettes, and burettes, is essential to ensure accurate measurements in the analytical laboratory. Calibration helps maintain consistency across measurements and prevents errors arising from inaccurate volumes.
Volumetric flasks: Volumetric flasks are designed to prepare aqueous solutions of precise volumes accurately with a calibration line on the neck. To calibrate a volumetric flask, it is important to fill it with distilled...
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Calibration by commutable control materials is able to reduce inter-method differences of current high-performance

Renata Paleari1, Ferruccio Ceriotti2, Cornelis L Harteveld3

  • 1Dip. di Fisiopatologia Medico-Chirurgica e dei Trapianti, Centro per la Riferibilità Metrologica in Medicina di Laboratorio (CIRME), Università degli Studi di Milano, Milano, Italy.

Clinica Chimica Acta; International Journal of Clinical Chemistry
|December 6, 2017
PubMed
Summary

Accurate hemoglobin A2 (HbA2) measurement is crucial. Using commutable control materials as calibrators significantly reduces variability between different laboratory methods, improving diagnostic accuracy.

Keywords:
CommutabilityHarmonizationHbA(2)Multicenter evaluationStandardizationβ-Thalassemia

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Area of Science:

  • Clinical Chemistry
  • Laboratory Medicine
  • Analytical Chemistry

Background:

  • Current methods for determining hemoglobin A2 (HbA2) show significant variability.
  • There is a need to compare high-performing HbA2 measurement techniques.
  • The commutability of available and developing control materials requires evaluation.

Purpose of the Study:

  • To compare different high-performance HbA2 measurement techniques.
  • To assess the commutability of various control materials for HbA2 determination.
  • To evaluate the impact of commutable controls on inter-method variability.

Main Methods:

  • Forty blood samples were analyzed in duplicate using HPLC and capillary electrophoresis systems.
  • Commutability of NIBSC WHO reagent, Bio-Rad Lyphochek, and lyophilized controls was assessed.
  • Controls were analyzed in quadruplicate alongside blood samples over two days.

Main Results:

  • Within-run imprecision for HbA2 measurement varied from 0.6% to 10.1%.
  • Methods showed high correlation (r=0.9941-0.9995) but significant bias.
  • Several control materials demonstrated poor commutability, impacting results.

Conclusions:

  • Adequate commutable control materials are essential for calibrating HbA2 measurement methods.
  • Using commutable controls can substantially reduce inter-method variability.
  • This reduction in variability brings routine methods closer to analytical goals based on biological variability.