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Author Spotlight: Advancing Tear Fluid Analysis Using a Standardized Protocol for Proteomics Research
Published on: December 1, 2023
Determination of olopatadine in human tears by hydrophilic interaction liquid chromatography-MS/MS method
Jelena Maksić1, Ana Stajić2, Miroslav Knežević3
1Department of Drug Control & Examination, Sector for Pharmacy, Military Medical Academy, Crnotravska 17, Belgrade, Serbia.
Aim:
The objective of the study was development of hydrophilic interaction liquid chromatography-ESI/MS/MS method for the determination of olopatadine in tear matrix.
Materials & Methods:
Separation was performed on Acquity BEH amide column (2.1 × 100 mm, 1.7 μm). The mobile phase was consisted of 0.1% formic acid in water and acetonitrile. Mianserin hydrochloride was implemented as an internal standard. The artificial tear fluid was used as matrix. The tear samples were collected using Schirmer test strips. For the optimization of ultra pressure liquid chromatography conditions, Box-Benhken design was utilized.
Results:
The optimal values of the ion source and collision cell parameters were found. Quantification was performed in multiple reaction monitoring mode. The optimized method was fully validated.
Conclusion:
The proposed method was utilized for monitoring of olopatadine in human tear.
