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Ionic signalling and mitochondrial dynamics

Nada Abuarab1, Fangfang Li1, Asipu Sivaprasadarao1,2

  • 1School of Biomedical Sciences, Faculty of Biological Sciences, LS2 9JT, Leeds, U.K.

Molecular & Cellular Oncology
|December 7, 2017
PubMed

Insights

Increased reactive oxygen species (ROS) fragment mitochondria in aging. Our study shows ROS-activated TRPM2 channels release lysosomal zinc, causing mitochondrial fragmentation, offering therapeutic insights.

Area of Science:

  • Cellular Biology
  • Mitochondrial Dynamics
  • Age-Related Diseases

Background:

  • Elevated intracellular reactive oxygen species (ROS) are implicated in age-related diseases.
  • Mitochondrial fragmentation is a hallmark of cellular aging and disease.
  • The precise mechanisms linking ROS to mitochondrial fragmentation require further elucidation.

Purpose of the Study:

  • To investigate the role of TRPM2 channels in ROS-induced mitochondrial fragmentation.
  • To elucidate the downstream signaling pathway involving lysosomal zinc release.
  • To explore potential therapeutic targets for age-related mitochondrial dysfunction.

Main Methods:

  • Utilized cellular models to study reactive oxygen species (ROS) signaling.
  • Investigated the function of TRPM2 channels using specific inhibitors and genetic approaches.
  • Assessed lysosomal zinc release and mitochondrial morphology via advanced microscopy techniques.

Main Results:

  • Demonstrated that ROS directly activate TRPM2 channels.
  • Showcased TRPM2 channel activation leading to lysosomal Zn2+ release.
  • Confirmed that lysosomal zinc triggers mitochondrial fragmentation.

Conclusions:

  • ROS-activated TRPM2 channels represent a critical link to mitochondrial fragmentation.
  • Lysosomal zinc release is a key mediator in this process.
  • Targeting the TRPM2-lysosomal-mitochondrial axis may offer therapeutic strategies for aging.
Keywords:
TRPM2calciumcell migrationcell proliferationlysosomal membrane permeabilisationmitochondrial dynamicsoxidative stressreactive oxygen specieszinc

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