Related Experiment Video
Updated: Feb 17, 2026

10:50
Using In Vitro Fluorescence Resonance Energy Transfer to Study the Dynamics Of Protein Complexes at a Millisecond Time Scale
Published on: March 14, 2019
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Lighting-up protein-ligand interactions with fluorescent PET (photoinduced electron transfer) sensor designs
Bernard McLaughlin1, Esther M Surender, Glenn D Wright
1School of Chemistry and Chemical Engineering, Queen's University, Belfast BT9 5AG, Northern Ireland, UK. a.desilva@qub.ac.uk.
Abstract:
The fluorescence of sensors 1 and 6, which are designed in terms of photoinduced electron transfer (PET), is switched 'on' by factors of 15 and 6 with avidin in a rather selective manner. Sensors 3 and 7, simpler derivatives of 1, are switched 'on' by factors of 16 and 7.5 with bovine serum albumin, though at 30-fold higher concentrations.

