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Updated: Feb 17, 2026

Microbiota Analysis Using Two-step PCR and Next-generation 16S rRNA Gene Sequencing
Published on: October 15, 2019
ASAR: visual analysis of metagenomes in R
Askarbek N Orakov1,2, Nazgul K Sakenova1,2, Anatoly Sorokin3,4
1Biological Systems Unit, Okinawa Institute of Science and Technology, Onna-son 904-0412, Japan.
Motivation:
Functional and taxonomic analyses are critical steps in understanding interspecific interactions within microbial communities. Currently, such analyses are run separately, which complicates interpretation of results. Here we present the ASAR interactive tool for simultaneous analysis of metagenomic data in three dimensions: taxonomy, function, metagenome.
Results:
An interactive data analysis tool for selection, aggregation and visualization of metagenomic data is presented. Functional analysis with a SEED hierarchy and pathway diagram based on KEGG orthology based upon MG-RAST annotation results is available.
Availability And Implementation:
Source code of the ASAR is accessible at GitHub (https://github.com/Askarbek-orakov/ASAR).
Contact:
askarbek.orakov@nu.edu.kz or goryanin@gmail.com.
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