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Particle Agglutination Method for Poliovirus Identification
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Diagnostic Assay Development for Poliovirus Eradication.

Nancy Gerloff1, Hong Sun1, Mark Mandelbaum1

  • 1Division of Viral Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia, USA.

Journal of Clinical Microbiology
|December 8, 2017
PubMed
Summary
This summary is machine-generated.

New molecular assays (ITD 5.0) enhance poliovirus surveillance by accurately differentiating wild and vaccine polioviruses. These real-time PCR assays are crucial for global poliovirus eradication efforts.

Keywords:
diagnostic assaymolecular typingpolio eradicationpoliovirusrRT-PCRreal-time reverse transcription-PCRserotype identification

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Area of Science:

  • Virology
  • Molecular Biology
  • Public Health

Background:

  • Global poliovirus eradication is nearing completion.
  • Intratypic differentiation (ITD) is vital for laboratory surveillance to distinguish wild from vaccine polioviruses.
  • Recent WPV1 variants and PV2 containment necessitated updates to ITD methods.

Purpose of the Study:

  • To describe the development and validation of ITD version 5.0.
  • To evaluate the performance of new real-time reverse transcription-PCR (rRT-PCR) assays for poliovirus detection and differentiation.
  • To establish limits of detection and specificities for ITD 5.0 assays.

Main Methods:

  • Development of 16 control RNA transcripts and nine plaque-isolated viruses as quantitation standards.
  • Validation of six ITD rRT-PCR assays using these standards.
  • Design and pilot testing of two new assays for WPV1 genotypes and all PV2 viruses.

Main Results:

  • ITD version 5.0 comprises six validated rRT-PCR assays.
  • New assays demonstrated high performance: WPV1 assay (99.1% specificity, 100% sensitivity) and PV2 assay (97.7% specificity, 92% sensitivity).
  • Limits of detection and specificities were determined for all assays.

Conclusions:

  • ITD version 5.0 provides accurate diagnostic tools for poliovirus surveillance.
  • The validated assays are essential for ongoing global poliovirus eradication.
  • Findings support future development of molecular diagnostic tools for infectious disease surveillance.