PD-L1 Prevents the Development of Autoimmune Heart Disease in Graft-versus-Host Disease

Effector memory T cells (T_EM) are less capable of inducing graft-versus-host disease (GVHD) compared with naive T cells (T_N). Previously, in the TS1 TCR transgenic model of GVHD, wherein TS1 CD4 cells specific for a model minor histocompatibility Ag (miHA) induce GVHD in miHA-positive recipients, we found that cell-intrinsic properties of TS1 T_EM reduced their GVHD potency relative to TS1 T_N Posttransplant, TS1 T_EM progeny expressed higher levels of PD-1 than did TS1 T_N progeny, leading us to test the hypothesis that T_EM induce less GVHD because of increased sensitivity to PD-ligands. In this study, we tested this hypothesis and found that indeed TS1 T_EM induced more severe skin and liver GVHD in the absence of PD-ligands. However, lack of PD-ligands did not result in early weight loss and colon GVHD comparable to that induced by TS1 T_N, indicating that additional pathways restrain alloreactive T_EM TS1 T_N also caused more severe GVHD without PD-ligands. The absence of PD-ligands on donor bone marrow was sufficient to augment GVHD caused by either T_EM or T_N, indicating that donor PD-ligand-expressing APCs critically regulate GVHD. In the absence of PD-ligands, both TS1 T_EM and T_N induced late-onset myocarditis. Surprisingly, this was an autoimmune manifestation, because its development required non-TS1 polyclonal CD8⁺ T cells. Myocarditis development also required donor bone marrow to be PD-ligand deficient, demonstrating the importance of donor APC regulatory function. In summary, PD-ligands suppress both miHA-directed GVHD and the development of alloimmunity-induced autoimmunity after allogeneic hematopoietic transplantation.