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Study on mechanism about long noncoding RNA MALAT1 affecting pancreatic cancer by regulating Hippo-YAP signaling

Yongping Zhou1, Ting Shan2, Wenzhou Ding1

  • 1Department of Hepatobiliary, Wuxi Second Hospital, Nanjing Medical University, Wuxi, Jiangsu, China.

Journal of Cellular Physiology
|December 8, 2017
PubMed

Insights

High expression of long non-coding RNA MALAT1 in pancreatic cancer (PC) promotes tumor growth and metastasis by inhibiting the Hippo-YAP signaling pathway. Silencing MALAT1 reduces proliferation and invasion, offering a potential therapeutic target.

Area of Science:

  • Oncology
  • Molecular Biology
  • Biochemistry

Background:

  • Pancreatic cancer (PC) is a highly aggressive malignancy with poor prognosis.
  • Long non-coding RNAs (lncRNAs) play critical roles in cancer development and progression.
  • The Hippo-YAP signaling pathway is frequently dysregulated in various cancers, including PC.

Purpose of the Study:

  • To investigate the molecular mechanism of lncRNA MALAT1 in pancreatic cancer.
  • To explore the role of lncRNA MALAT1 in regulating the Hippo-YAP signaling pathway.
  • To assess the impact of lncRNA MALAT1 on cancer cell proliferation, apoptosis, migration, and invasion.

Main Methods:

  • Quantitative real-time PCR (qRT-PCR) and Western blot to detect MALAT1, LATS1, and YAP1 expression.
  • Cell Counting Kit-8 (CCK-8) assay for proliferation.
  • Flow cytometry for apoptosis.
  • Wound healing and Transwell assays for migration and invasion.
  • Immunohistochemistry and in vivo tumor xenograft models.

Main Results:

  • lncRNA MALAT1 was highly expressed in PC tissues and cells.
  • Silencing MALAT1 (si-MALAT1) decreased proliferation, induced apoptosis, and reduced migration and invasion of PC cells (AsPC-1).
  • MALAT1 dysregulated LATS1 (down-regulated) and YAP1 (up-regulated) expression within the Hippo-YAP pathway, leading to increased tumor growth and Ki-67 expression in vivo.

Conclusions:

  • High expression of lncRNA MALAT1 in pancreatic cancer promotes tumor progression.
  • lncRNA MALAT1 influences proliferation, apoptosis, migration, and invasion by modulating the Hippo-YAP signaling pathway.
  • lncRNA MALAT1 represents a potential therapeutic target for pancreatic cancer treatment.
Keywords:
Hippo-YAP signalinglncRNA MALAT1pancreatic cancer

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