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Micro-competition system for Raman quantification of multiple glycans on intact cell surface
Yunlong Chen1, Lin Ding1, Junqiang Xu1
1State Key Laboratory of Analytical Chemistry for Life Science , School of Chemistry and Chemical Engineering , Nanjing University , Nanjing 210093 , P.R. China . Email: hxju@nju.edu.cn ; ; Tel: +86 25 83593593.
Chemical Science
|December 9, 2017
Summary
This study introduces a novel micro-competition system for quantifying multiple cell surface glycans simultaneously. The innovative method uses gold nanoprobes and surface-enhanced Raman scattering (SERS) for precise glycan analysis.
Area of Science:
- Biochemistry and analytical chemistry
- Nanotechnology and materials science
- Cell biology and glycobiology
Background:
- Cell surface glycans play crucial roles in biological processes.
- Accurate quantification of multiple glycans on intact cells remains challenging.
- Existing methods often lack the multiplexing capability for complex glycan analysis.
Purpose of the Study:
- To develop a micro-competition system for simultaneous quantification of multiple glycans on intact cell surfaces.
- To integrate two-surface-one-molecule competition with surface-enhanced Raman scattering (SERS) for enhanced detection.
- To provide a powerful tool for investigating complex glycan-related biological processes.
Main Methods:
- Designed a micro-competition system involving polysaccharide-coated gold nanostars, target cells, and functionalized gold nanoprobes.
- Utilized gold nanoprobes coated with distinct Raman molecules and lectins for signal resolution and glycan recognition.
- Employed silica bubbles as artificial glycan surfaces and SERS substrates for competitive recognition and capture of nanoprobes.
- Simultaneously detected gold nanoprobes on bubbles using SERS to quantify corresponding cell surface glycans.
Main Results:
- Achieved simultaneous quantification of multiple glycans on intact cell surfaces.
- Demonstrated specific capture of gold nanoprobes by cells and bubbles based on lectin-glycan recognition.
- Validated the micro-competition system's capability for high-throughput glycan analysis.
- Showcased the system's potential for investigating complex glycan-mediated biological interactions.
Conclusions:
- The developed micro-competition system enables simultaneous, high-sensitivity quantification of multiple cell surface glycans.
- This approach offers a powerful new tool for advancing research in glycobiology and cell surface analysis.
- The system facilitates deeper understanding of the roles of complex glycans in various biological processes.
