Related Experiment Video
Updated: Feb 17, 2026

Glucose Uptake Measurement and Response to Insulin Stimulation in In Vitro Cultured Human Primary Myotubes
Published on: June 25, 2017
Glucose Transport Activity Measured in Giant Vesicles
Jesper S Hansen1, Karin Lindkvist-Petersson2
1Department of Experimental Medical Science, Lund University, BMC C13, 221 84, Lund, Sweden. jesper.sondergaard_hansen@med.lu.se.
Abstract:
Incorporation of membrane proteins and internal reporter systems directly into giant vesicles, during their formation from a hydrogel surface, has emerged as a promising new concept in membrane protein characterization. Here, we provide the detailed protocol for a glucose transporter activity assay based on giant vesicles containing a fluorescent enzyme-linked reporter system internally. This assay is applicable for the functional analysis of a variety of hexose-transporting proteins. We furthermore believe that it can aid in the development of drugs targeting hexose transporters.
More Related Videos
Related Concept Videos
Membrane Proteins
Glucose Transporters
Facilitated diffusion-glucose transporters (GLUTs) are encoded by the solute-linked carrier (SLC) family 2, subfamily A gene family, or SLC2A. The 14 GLUT protein members are distributed into three classes:
Glucose Absorption Into the Small Intestine
Secondary Active Transport
Secondary Active Transport
Insulin Secretory Vesicles

