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Tracking GLUT2 Translocation by Live-Cell Imaging.

Sabina Tsytkin-Kirschenzweig1, Merav Cohen1,2, Yaakov Nahmias3,4

  • 1Alexander Grass Center for Bioengineering, The Rachel and Selim Benin School of Computer Science and Engineering, The Hebrew University of Jerusalem, Jerusalem, 9190401, Israel.

Methods in Molecular Biology (Clifton, N.J.)
|December 9, 2017
PubMed
Summary
This summary is machine-generated.

Researchers developed a live imaging system to track glucose transporter 2 (GLUT2) movement in cells. This tool aids in discovering drugs for metabolic disorders and kidney diseases linked to GLUT2 dysfunction.

Keywords:
GLUT2Glucose transportKidney functionLive imagingPolarized epitheliumTranslocationType 2 diabetes

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Area of Science:

  • Cell Biology
  • Metabolic Biochemistry
  • Molecular Medicine

Background:

  • The facilitative glucose transporter (GLUT) family is crucial for metabolic homeostasis.
  • GLUT2 is uniquely expressed in key metabolic organs: intestine, pancreas, liver, and kidney.
  • GLUT2 dysfunction is implicated in pathologies like Fanconi-Bickel syndrome, causing growth and renal issues.

Purpose of the Study:

  • To establish a live imaging system for visualizing and quantifying GLUT2 translocation dynamics.
  • To enable the testing of potential modulators of GLUT2 membrane translocation.
  • To identify novel therapeutic targets for impaired glucose homeostasis and associated nephropathies.

Main Methods:

  • Development of a live imaging system using a mCherry-hGLUT2 fusion protein.
  • Utilizing polarized epithelial cells to mimic physiological conditions.
  • Quantification of GLUT2 translocation and its dynamics in real-time.

Main Results:

  • Successfully established a system to visualize and quantify GLUT2 translocation.
  • Demonstrated the ability to study GLUT2 dynamics in polarized epithelial cells.
  • Created a platform for screening GLUT2 translocation modulators.

Conclusions:

  • Live imaging of mCherry-hGLUT2 provides a powerful tool to study transporter dynamics.
  • This system facilitates the discovery of drugs targeting GLUT2 for metabolic and renal diseases.
  • Understanding GLUT2 translocation is key to addressing glucose homeostasis disorders.