Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Liver Regeneration

Liver Regeneration

The liver is an important organ in vertebrates that plays an essential role in metabolism. It is also responsible for storing and redistributing nutrients such as carbohydrates, fats, and vitamins in the body. Additionally, the liver releases bile salts which are critical for digesting food and eliminating toxic metabolites from the body.
Cells of Liver
The liver comprises four major types of cells— hepatocytes, stellate, Kupffer, and sinusoidal endothelial cells. The hepatocytes are...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Composite Liquid Marble Templated Millimetric Capsule With Tunable Rigidity, Porosity, and Thermal Reconfigurability Toward 3D Cell Culture.

Advanced materials (Deerfield Beach, Fla.)·2026

Same author

Active endothelial and neural regulation of valve biology, health, and disease.

Frontiers in cardiovascular medicine·2026

Same author

Engineered GelMA Microgels for Locoregional Doxorubicin Release and Apoptosis Induction in Oral Squamous Cell Carcinoma.

ACS biomaterials science & engineering·2026

Same author

Bioengineered In Vitro 3D Cancer Models: A New Paradigm in Ethical and Predictive Oncology Research Toward Successful Pre-Clinical Drug Screening.

Small (Weinheim an der Bergstrasse, Germany)·2026

Same author

Valve endothelial monolayer fissuring and interstitial matrix fracture triggers calcific lesion formation via GTPase signaling.

Acta biomaterialia·2026

Same author

Osteosarcoma-on-a-chip model mimicking intra-tumoral heterogeneity to interrogate tumor-associated macrophage reprogramming for immunotherapeutics.

Biomaterials·2025

Search research articles

Related Experiment Video

Updated: Feb 17, 2026

Bioprinting Cellularized Constructs Using a Tissue-specific Hydrogel Bioink

Bioprinting Cellularized Constructs Using a Tissue-specific Hydrogel Bioink

Published on: April 21, 2016

Functional hepatocyte clusters on bioactive blend silk matrices towards generating bioartificial liver constructs.

G Janani¹, Samit K Nandi², Biman B Mandal¹

¹Biomaterials and Tissue Engineering Laboratory, Department of Biosciences and Bioengineering, Indian Institute of Technology Guwahati, Guwahati 781039, Assam, India.

Acta Biomaterialia

|December 11, 2017

Summary

Researchers developed a novel silk blend scaffold to create functional in vitro liver tissue. This bio-matrix supports hepatocyte growth and enhances liver-specific functions, offering promise for bioartificial liver devices and regenerative medicine.

Keywords:

Bioartificial liver Hepatocytes Non-mulberry silk Silk Tissue engineering

More Related Videos

Three-Dimensional Collagen Matrix Scaffold Implantation as a Liver Regeneration Strategy

Three-Dimensional Collagen Matrix Scaffold Implantation as a Liver Regeneration Strategy

Published on: June 29, 2021

In Vitro Cultivation Techniques for Modeling Liver Organogenesis, Building Assembloids, and Designing Synthetic Tissues using Human Cell Lines

In Vitro Cultivation Techniques for Modeling Liver Organogenesis, Building Assembloids, and Designing Synthetic Tissues using Human Cell Lines

Published on: April 18, 2025

Related Experiment Videos

Last Updated: Feb 17, 2026

Bioprinting Cellularized Constructs Using a Tissue-specific Hydrogel Bioink

Bioprinting Cellularized Constructs Using a Tissue-specific Hydrogel Bioink

Published on: April 21, 2016

Three-Dimensional Collagen Matrix Scaffold Implantation as a Liver Regeneration Strategy

Three-Dimensional Collagen Matrix Scaffold Implantation as a Liver Regeneration Strategy

Published on: June 29, 2021

In Vitro Cultivation Techniques for Modeling Liver Organogenesis, Building Assembloids, and Designing Synthetic Tissues using Human Cell Lines

In Vitro Cultivation Techniques for Modeling Liver Organogenesis, Building Assembloids, and Designing Synthetic Tissues using Human Cell Lines

Published on: April 18, 2025

Area of Science:

Biomaterials Science
Tissue Engineering
Regenerative Medicine

Background:

Developing in vitro functional hepatic tissue is crucial for bioartificial liver (BAL) and tissue engineering applications.
Cirrhosis impairs liver function, increasing demand for functional in vitro liver constructs due to donor scarcity.
Silk fibroin from mulberry (Bombyx mori) and non-mulberry (Antheraea assamensis) silks possess distinct properties influencing cell behavior.

Purpose of the Study:

To investigate a novel blend (BA) silk scaffold for generating functional liver constructs.
To evaluate the potential of blending mulberry and RGD-rich non-mulberry silk fibroin for hepatic tissue engineering.
To assess the scaffold's ability to support hepatocyte growth, interaction, and liver-specific functions.

Main Methods:

Fabrication of three-dimensional (3D) porous silk scaffolds: mulberry (BM), non-mulberry (AA), and blend (BA).
Physicochemical characterization and functional evaluation using HepG2 and primary neonatal rat hepatocytes.
Assessment of cell growth, distribution, albumin/urea synthesis, and cytochrome P450 activity over 21 days.

Main Results:

Hemocompatible BA scaffolds promoted high-density hepatocyte cluster formation, enhancing cell-matrix and cell-cell interactions.
Blend scaffolds significantly improved liver-specific functions (albumin, urea synthesis, CYP450 activity) of cultured hepatocytes.
Subcutaneous implantation showed minimal macrophage infiltration in blend scaffolds, indicating good biocompatibility.

Conclusions:

The integral properties of the blend (BA) scaffold provide a suitable niche for spheroidal hepatocyte growth and enhanced biological activity.
This novel 3D bio-matrix supports functional liver cell growth with future prospects in BAL and regenerative medicine.
The blend scaffold effectively mimics the native liver micro-environment, improving hepatocyte function in vitro.