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Related Concept Videos

RNA-seq03:21

RNA-seq

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Measuring Nascent Transcripts by Nascent-seq.

Fei Xavier Chen1, Stacy A Marshall2, Yu Deng2

  • 1Department of Biochemistry and Molecular Genetics, Feinberg School of Medicine, Northwestern University, Searle 6-510, 320 E. Superior St., Chicago, IL, 60611, USA. Fei.X.Chen@u.northwestern.edu.

Methods in Molecular Biology (Clifton, N.J.)
|December 11, 2017
PubMed
Summary
This summary is machine-generated.

Researchers developed a new transcriptome-wide method to rapidly measure nascent transcribing RNA. This approach provides an unbiased way to visualize polymerase progress and quantify RNA transcripts genome-wide.

Keywords:
Nascent-seqNext-generation sequencingPol IITranscriptionmRNA

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Area of Science:

  • Molecular Biology
  • Genomics
  • Transcriptomics

Background:

  • Understanding transcription and RNA processing is crucial for gene expression regulation.
  • Existing methods for genome-wide analysis of nascent RNA are often limited in speed and scope.

Purpose of the Study:

  • To present a novel transcriptome-wide method for measuring nascent transcribing RNA.
  • To enable fast and unbiased quantification of nascent RNA at a genome-wide scale.

Main Methods:

  • Development of a new transcriptome-wide technique.
  • Measurement of nascent transcribing RNA levels.
  • Genome-wide analysis of RNA polymerase activity.

Main Results:

  • A fast and unbiased method for quantifying nascent RNA was established.
  • The method allows for genome-wide assessment of transcription levels.
  • Provides insights into polymerase progress and co-transcriptional processing.

Conclusions:

  • The presented method offers a robust approach for studying transcription.
  • Facilitates a deeper understanding of genome-wide transcription and RNA processing events.