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Maxam-Gilbert Sequencing

Maxam-Gilbert Sequencing

In the same year as the discovery of the Sanger sequencing method, another group of scientists, Allan Maxam and Walter Gilbert, demonstrated their chemical-cleavage method for DNA sequencing. The Maxam-Gilbert method relies on using different chemicals that can cleave the DNA sequence at specific sites, the separation of resulting DNA fragments of variable size using electrophoresis, and deciphering the DNA sequence from the resulting gel bands.
Challenges of the Maxam-Gilbert Method
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Updated: Feb 17, 2026

Comprehensive DNA Methylation Analysis Using a Methyl-CpG-binding Domain Capture-based Method in Chronic Lymphocytic Leukemia Patients

Comprehensive DNA Methylation Analysis Using a Methyl-CpG-binding Domain Capture-based Method in Chronic Lymphocytic Leukemia Patients

Published on: June 16, 2017

Methyl-CpG-Binding Domain Sequencing: MBD-seq.

Karolina A Aberg¹, Robin F Chan¹, Linying Xie¹

¹Center for Biomarker Research and Precision Medicine, Virginia Commonwealth University, P. O. Box 980533, Richmond, VA, 23298, USA.

Methods in Molecular Biology (Clifton, N.J.)

|December 11, 2017

Summary

This summary is machine-generated.

Methylome-wide association studies (MWAS) are crucial for understanding common diseases. Methyl-CpG-binding domain sequencing (MBD-seq) provides a cost-effective method for analyzing the methylome, identifying disease-associated methylation sites.

Keywords:

Affinity-based capture Blood spots CpG Differentially methylated regions High-dimensional data analysis MBD-seq MWAS Methyl-CpG-binding domain MethylMiner Methylome-wide association studies RaMWAS Sequencing

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Last Updated: Feb 17, 2026

Comprehensive DNA Methylation Analysis Using a Methyl-CpG-binding Domain Capture-based Method in Chronic Lymphocytic Leukemia Patients

Comprehensive DNA Methylation Analysis Using a Methyl-CpG-binding Domain Capture-based Method in Chronic Lymphocytic Leukemia Patients

Published on: June 16, 2017

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Published on: October 31, 2016

Enhanced Reduced Representation Bisulfite Sequencing for Assessment of DNA Methylation at Base Pair Resolution

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Published on: February 24, 2015

Area of Science:

Epigenetics and Genomics
Disease Biomarker Discovery

Background:

Limited understanding of the methylome's role in common diseases hinders biomarker identification.
Methylome-wide association studies (MWAS) are essential for detecting disease-relevant methylation sites.

Purpose of the Study:

To evaluate Methyl-CpG-binding domain sequencing (MBD-seq) as an efficient method for MWAS.
To demonstrate the feasibility of MBD-seq for large-scale methylome analysis.

Main Methods:

Optimization of the Methyl-CpG-binding domain sequencing (MBD-seq) protocol.
Comparison of MBD-seq performance against whole-genome bisulfite sequencing (WGBS).

Main Results:

An optimized MBD-seq protocol achieves sensitivity and specificity comparable to WGBS.
MBD-seq offers significant cost and time savings compared to WGBS.
MBD-seq is economically viable for the sample sizes required for MWAS.

Conclusions:

MBD-seq is a powerful and cost-effective tool for comprehensive methylome profiling in MWAS.
This method facilitates the discovery of methylation sites associated with common diseases.