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Methyl-binding DNA capture Sequencing for Patient Tissues
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Targeted Bisulfite Sequencing Using the SeqCap Epi Enrichment System.

Jennifer Wendt1, Heidi Rosenbaum1, Todd A Richmond1

  • 1Roche Sequencing Solutions, 500 S. Rosa Road, Madison, WI, 53719, USA.

Methods in Molecular Biology (Clifton, N.J.)
|December 11, 2017
PubMed
Summary
This summary is machine-generated.

This study introduces a targeted DNA enrichment method for precise cytosine methylation analysis. It enables cost-effective, high-resolution sequencing of specific genomic regions, aiding in disease research.

Keywords:
BisulfiteHybridizationNGSSeqCap EpiSequencingTargeted enrichment

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Area of Science:

  • Epigenetics
  • Genomics
  • Molecular Biology

Background:

  • Cytosine methylation is crucial for mammalian development and implicated in diseases like cancer.
  • Whole Genome Shotgun Bisulfite Sequencing (WGBS) is effective but costly and time-consuming for targeted analysis.
  • Researchers often need high-resolution methylation data for specific genomic subsets.

Purpose of the Study:

  • To present a cost-effective method for targeted high-resolution cytosine methylation analysis.
  • To demonstrate the utility of SeqCap Epi for enriching bisulfite-treated DNA.
  • To enable efficient sequencing of specific genomic regions for methylation studies.

Main Methods:

  • Utilized SeqCap Epi technology for targeted enrichment of bisulfite-treated DNA.
  • Applied next-generation sequencing to focused genomic subsets.
  • Employed the SeqCap Epi CpGiant design to target millions of CpGs in the human genome.
  • Developed a multiplexing strategy for high-throughput sample processing.

Main Results:

  • The procedure allows for high-resolution cytosine methylation analysis on targeted genomic regions (kb to >200 Mb).
  • SeqCap Epi effectively enriches bisulfite-treated DNA for subsequent sequencing.
  • The method supports multiplexing, enabling cost-effective analysis of numerous samples.

Conclusions:

  • Targeted enrichment via SeqCap Epi provides an efficient and affordable approach for high-resolution DNA methylation studies.
  • This method facilitates focused investigation of epigenetic alterations in specific genomic regions.
  • The technique is valuable for research into diseases associated with aberrant cytosine methylation patterns.