Virus Binding and Internalization Assay for Adeno-associated Virus.
Garrett E Berry1,2, Longping V Tse1
1Gene Therapy Center, The University of North Carolina at Chapel Hill, Chapel Hill, NC, USA.
Bio-Protocol
|December 12, 2017
Summary
This study presents a quantitative PCR (qPCR) method to measure adeno-associated virus (AAV) binding and internalization. This technique aids in understanding viral entry mechanisms and can be adapted for other viruses.
Area of Science:
- Virology
- Molecular Biology
- Cell Biology
Background:
- Adeno-associated virus (AAV) binding and internalization are critical for its infectivity and tropism.
- Dissecting these processes offers insights into viral entry and trafficking pathways.
Purpose of the Study:
- To describe a quantitative PCR (qPCR)-based protocol for measuring AAV cell surface binding and internalization.
- To provide a method for quantifying viral genome uptake to understand virus-cell interactions.
Main Methods:
- Development of a qPCR assay for viral genome quantification.
- Differentiation between cell-surface bound AAV and internalized AAV.
Main Results:
- The protocol allows for the quantitative assessment of AAV binding and internalization.
- Viral genome quantification via qPCR provides a measure of virus uptake.
Conclusions:
- The described qPCR method is effective for studying AAV entry dynamics.
- This protocol can serve as a foundation for investigating entry mechanisms of other viruses.
