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Dynamic content exchange between liprotides.

Henriette S Frislev1, Stine C L Jakobsen1, Signe A Frank1

  • 1Interdisciplinary Nanoscience Center (iNANO), Department of Molecular Biology and Genetics, Aarhus University, Gustav Wieds Vej 14, DK - 8000 Aarhus, Denmark.

Biophysical Chemistry
|December 12, 2017
PubMed
Summary

Liprotides rapidly transfer fatty acids to cell membranes, destabilizing them to kill tumor cells. This rapid transfer highlights their potential as effective transporters for hydrophobic compounds.

Keywords:
DynamicsLiprotidesMembrane transferPyrene

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Area of Science:

  • Biochemistry
  • Cell Biology
  • Biophysics

Background:

  • Liprotides are protein-fatty acid complexes with tumor cell-killing properties.
  • They are similar to the HAMLET complex, utilizing partially denatured proteins like alpha-lactalbumin (aLA) and fatty acids such as oleic acid (OA).

Purpose of the Study:

  • To investigate the dynamics of content transfer between liprotides and cell membranes.
  • To understand the kinetics of this transfer process and its dependence on membrane composition.

Main Methods:

  • Utilized liprotides composed of alpha-lactalbumin (aLA) and oleic acid (OA).
  • Employed the hydrophobic fluorescent probe pyrene to track transfer dynamics.
  • Used a stopped-flow instrument to measure rapid transfer kinetics.
  • Investigated the effect of cholesterol on transfer rates.

Main Results:

  • Pyrene exchange between liprotides occurred within the dead time of the stopped-flow instrument, indicating extremely rapid internal exchange.
  • Transfer of pyrene from liprotides to membranes occurred within 20 seconds.
  • Cholesterol, a membrane-stabilizing lipid, did not affect the transfer kinetics.

Conclusions:

  • Liprotides exhibit remarkably rapid transfer of their components to cell membranes.
  • This rapid transfer mechanism underscores the efficacy of liprotides as transporters of hydrophobic compounds.
  • The findings provide insights into the mechanism of liprotide-mediated cytotoxicity.