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Updated: May 21, 2026

Dissipative Microgravimetry to Study the Binding Dynamics of the Phospholipid Binding Protein Annexin A2 to Solid-supported Lipid Bilayers Using a Quartz Resonator
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Published on: November 1, 2018

Quartz Crystal Microbalances as Tools for Probing Protein-Membrane Interactions.

Søren B Nielsen1, Daniel E Otzen2

  • 1Interdisciplinary Nanoscience Center (iNANO), Aarhus University, Gustav Wieds Vej 14, DK - 8000, Aarhus C, Denmark.

Methods in Molecular Biology (Clifton, N.J.)
|May 19, 2026
PubMed
Summary
This summary is machine-generated.

Supported lipid bilayers (SLBs) mimic cell membranes and are formed using vesicle collapse. Quartz crystal microbalance with dissipation monitoring (QCM-D) enables real-time analysis of biomolecular interactions with SLBs.

Keywords:
InteractionQuartz crystal microbalance with dissipation (QCM-D)SiO2Supported lipid bilayer

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Au-Interaction of Slp1 Polymers and Monolayer from Lysinibacillus sphaericus JG-B53 - QCM-D, ICP-MS and AFM as Tools for Biomolecule-metal Studies

Published on: January 19, 2016

Area of Science:

  • Biophysics
  • Materials Science
  • Biochemistry

Background:

  • Supported lipid bilayers (SLBs) are widely used model systems for cell membranes.
  • Vesicle collapse is a common method for SLB formation on diverse substrates.
  • Quartz crystal microbalance with dissipation monitoring (QCM-D) is crucial for studying SLB formation and interactions.

Purpose of the Study:

  • To present a reliable method for forming SLBs using zwitterionic and charged lipids on SiO2 sensor crystals.
  • To enable real-time investigation of protein and peptide interactions with these SLBs.

Main Methods:

  • Utilizing the spontaneous collapse of phospholipid vesicles.
  • Employing quartz crystal microbalance with dissipation monitoring (QCM-D) for real-time analysis.
  • Forming SLBs on SiO2 sensor crystals with various lipid compositions.

Main Results:

  • Demonstrated a robust procedure for SLB formation on SiO2 sensor crystals.
  • Established a platform for probing biomolecular interactions with SLBs in real time.
  • Showcased the versatility of SLBs for studying interactions with proteins and peptides.

Conclusions:

  • The developed procedure offers a versatile approach for creating functional SLBs.
  • QCM-D is an effective technique for characterizing SLBs and their interactions.
  • This method facilitates the study of membrane protein and peptide interactions within a cell membrane model.