Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

CRISPR/Cas9 Genome Editing01:28

CRISPR/Cas9 Genome Editing

2.0K
The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
2.0K
Conservative Site-specific Recombination and Phase Variation02:53

Conservative Site-specific Recombination and Phase Variation

6.9K
Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
The recognition sites for Cre recombinase called LoxP...
6.9K
CRISPR01:59

CRISPR

58.0K
Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
58.0K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Wastewater surveillance reveals patterns of antibiotic resistance across the United States.

Nature communications·2026
Same author

Pathogen nucleic acids data in wastewater solids from 147 treatment plants in the United States: 2024-2025.

Data in brief·2026
Same author

Detection of Monkeypox Virus Clade Ib DNA in Wastewater Solids at Wastewater Treatment Plants, United States.

Emerging infectious diseases·2025
Same author

1206 genomes reveal origin and movement of <i>Aedes aegypti</i> driving increased dengue risk.

Science (New York, N.Y.)·2025
Same author

Antimicrobial Resistance in Bovine Respiratory Disease Pathogens: A Systematic Review and Analysis of the Published Literature.

Animals : an open access journal from MDPI·2025
Same author

Wastewater surveillance reveals patterns of antibiotic resistance across the United States.

medRxiv : the preprint server for health sciences·2025

Related Experiment Video

Updated: Feb 17, 2026

Indel Detection following CRISPR/Cas9 Mutagenesis using High-resolution Melt Analysis in the Mosquito Aedes aegypti
05:30

Indel Detection following CRISPR/Cas9 Mutagenesis using High-resolution Melt Analysis in the Mosquito Aedes aegypti

Published on: September 10, 2021

3.8K

Highly Efficient Site-Specific Mutagenesis in Malaria Mosquitoes Using CRISPR.

Ming Li1, Omar S Akbari2, Bradley J White1

  • 1Department of Entomology, University of California, Riverside, California 92521 mingli@ucsd.edu bradwhite@google.com.

G3 (Bethesda, Md.)
|December 14, 2017
PubMed
Summary

Researchers developed a CRISPR/Cas9 gene-editing tool for malaria-carrying Anopheles mosquitoes. This breakthrough enables rapid creation of mutant mosquito lines for studying malaria transmission and control.

Keywords:
AnophelesCRISPRCas9gene drivereverse geneticstransgenics

More Related Videos

Preparing and Injecting Embryos of Culex Mosquitoes to Generate Null Mutations using CRISPR/Cas9
07:45

Preparing and Injecting Embryos of Culex Mosquitoes to Generate Null Mutations using CRISPR/Cas9

Published on: September 10, 2020

7.9K
Genome Editing in the Yellow Fever Mosquito Aedes aegypti using CRISPR-Cas9
06:47

Genome Editing in the Yellow Fever Mosquito Aedes aegypti using CRISPR-Cas9

Published on: March 21, 2025

2.0K

Related Experiment Videos

Last Updated: Feb 17, 2026

Indel Detection following CRISPR/Cas9 Mutagenesis using High-resolution Melt Analysis in the Mosquito Aedes aegypti
05:30

Indel Detection following CRISPR/Cas9 Mutagenesis using High-resolution Melt Analysis in the Mosquito Aedes aegypti

Published on: September 10, 2021

3.8K
Preparing and Injecting Embryos of Culex Mosquitoes to Generate Null Mutations using CRISPR/Cas9
07:45

Preparing and Injecting Embryos of Culex Mosquitoes to Generate Null Mutations using CRISPR/Cas9

Published on: September 10, 2020

7.9K
Genome Editing in the Yellow Fever Mosquito Aedes aegypti using CRISPR-Cas9
06:47

Genome Editing in the Yellow Fever Mosquito Aedes aegypti using CRISPR-Cas9

Published on: March 21, 2025

2.0K

Area of Science:

  • Vector Biology
  • Genetics
  • Molecular Biology

Background:

  • Anopheles mosquitoes transmit over 200 million malaria cases annually.
  • Understanding Anopheles biology is crucial for malaria control but limited by a lack of reverse genetics tools.

Purpose of the Study:

  • To report the successful application of the CRISPR/Cas9 system for gene editing in Anopheles mosquitoes.
  • To enable rapid creation of stable mutant lines for reverse genetic analysis.

Main Methods:

  • CRISPR/Cas9 system delivery via injection of guide RNAs (gRNAs) and Cas9 protein.
  • High concentration injection to achieve efficient, site-specific mutagenesis.
  • Application in diverse malaria vectors: Anopheles albimanus, A. coluzzii, and A. funestus.

Main Results:

  • Highly efficient and site-specific mutagenesis achieved in target Anopheles species.
  • Germline mutations were common and usually biallelic upon high-concentration injection.
  • Established a protocol for rapid generation of stable mutant Anopheles lines.

Conclusions:

  • The CRISPR/Cas9 protocol facilitates reverse genetics in key Anopheles malaria vectors.
  • Enables dissection of molecular and cellular basis of traits critical to malaria transmission.
  • Potential to identify novel targets for malaria control strategies.