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Updated: Feb 16, 2026

15N CPMG Relaxation Dispersion for the Investigation of Protein Conformational Dynamics on the µs-ms Timescale
Published on: April 19, 2021
Extracting unresolved coupling constants from complex multiplets by a real-time J-upscaled SERF experiment
Kathrin Buchberger1, Martin Walenta1, Klaus Zangger1
1Institute of Chemistry/Organic and Bioorganic Chemistry, University of Graz, Heinrichstrasse 28, A-8010, Graz, Austria.
Abstract:
The measurement of small homonuclear coupling constants is often prevented by either their small size and/or overlap with other signal splittings. Here, we present a real-time method to extract such couplings without interference from other splittings, with a resolution that is beyond conventional NMR spectra. In this real-time J-upscaled SERF experiment, homonuclear coupling is removed by slice-selective pure shift NMR, whereas scalar coupling to only one selected signal is reintroduced by selective refocusing. The remaining couplings are enhanced by real-time J-upscaling during interruptions of the FID data acquisition. The resulting spectrum is not only simplified by the restriction of the scalar coupling but also its resolution enhanced. This improved resolution results from a reduction of signal broadening due to magnetic field inhomogeneities from 2 different sources: slice-selective excitation and the spin-echo type J-upscaling element.
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