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Related Concept Videos

2D NMR: Overview of Heteronuclear Correlation Techniques01:18

2D NMR: Overview of Heteronuclear Correlation Techniques

817
Heteronuclear correlation spectroscopy is an analytical technique that investigates the coupling between different types of nuclei, often a proton and an X-nucleus, such as carbon-13 or nitrogen-15. This method is commonly used in nuclear magnetic resonance (NMR) spectroscopy to gain insights into complex chemical compounds' structural and compositional aspects. A typical heteronuclear correlation spectrum displays X-nucleus chemical shifts on one axis and a proton spectrum on the other...
817
2D NMR: Overview of Homonuclear Correlation Techniques01:16

2D NMR: Overview of Homonuclear Correlation Techniques

688
Homonuclear correlation spectroscopy (COSY) is a powerful technique used in Nuclear Magnetic Resonance (NMR) spectroscopy to study the correlations between nuclei of the same type within a molecule. It provides information about scalar couplings between adjacent nuclei, which helps determine connectivity and structural information. There are several COSY variants, each with its unique strengths and experimental parameters.
COSY90 is the standard two-dimensional (2D) COSY experiment that...
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¹³C NMR: ¹H–¹³C Decoupling01:04

¹³C NMR: ¹H–¹³C Decoupling

1.9K
The probability of having two carbon-13 atoms next to each other is negligible because of the low natural abundance of carbon-13. Consequently, peak splitting due to carbon-carbon spin-spin coupling is not observed in spectra. However, protons up to three sigma bonds away split the carbon signal according to the n+1 rule, resulting in complicated spectra.
A broadband decoupling technique is used to simplify these complex, sometimes overlapping, signals. Broadband decoupling relies on a...
1.9K
2D NMR: Heteronuclear Single-Quantum Correlation Spectroscopy (HSQC)01:19

2D NMR: Heteronuclear Single-Quantum Correlation Spectroscopy (HSQC)

1.5K
Heteronuclear single-quantum correlation spectroscopy (HSQC) is a 2D NMR technique that reveals one-bond correlations between hydrogen and a heteronucleus. The HSQC experiment is similar to the heteronuclear correlation experiment (HETCOR) but is more sensitive. In the HSQC spectrum, the proton chemical shift is plotted on the horizontal F2 axis, while the 13C chemical shift is plotted on the vertical F1 axis. The corresponding proton and 13C spectra are also shown. The HSQC contour plot does...
1.5K
2D NMR: Homonuclear Correlation Spectroscopy (COSY)01:06

2D NMR: Homonuclear Correlation Spectroscopy (COSY)

2.0K
Homonuclear correlation spectroscopy, or COSY, is a 2-dimensional NMR technique that provides information about coupled protons. Typically, the geminal and vicinal coupling are observed. For example, consider the COSY spectrum of ethyl acetate, where its 1D proton NMR spectrum is plotted along the vertical and horizontal axes with their corresponding chemical shift scale. Three spots on the diagonal corresponding to the three peaks in the 1D proton spectrum are called diagonal peaks. The COSY...
2.0K
Double Resonance Techniques: Overview01:12

Double Resonance Techniques: Overview

789
Double resonance techniques in Nuclear Magnetic Resonance (NMR) spectroscopy involve the simultaneous application of two different frequencies or radiofrequency pulses to manipulate and observe two distinct nuclear spins. One important application of double resonance is spin decoupling, which selectively suppresses coupling with one type of nucleus while observing the NMR signal from another nucleus, simplifying the spectrum and enhancing resolution.
Spin decoupling is usually achieved by...
789

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Updated: Feb 16, 2026

Measuring Interactions of Globular and Filamentous Proteins by Nuclear Magnetic Resonance Spectroscopy NMR and Microscale Thermophoresis MST
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Broadband homonuclear decoupled HSQMBC methods.

István Timári1,2, Katalin E Kövér1

  • 1Department of Inorganic and Analytical Chemistry, University of Debrecen, Egyetem tér 1, H-4032, Debrecen, Hungary.

Magnetic Resonance in Chemistry : MRC
|December 15, 2017
PubMed
Summary

A new real-time Zangger-Sterk CPMG-HSQMBC method precisely measures multiple-bond heteronuclear couplings. This technique enhances sensitivity and reduces experimental time for molecular analysis.

Keywords:
HSQMBCNMRPSYCHEZangger-Sterkbroadband homonuclear decouplingheteronuclear couplingspure shift

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Area of Science:

  • Nuclear Magnetic Resonance Spectroscopy
  • Organic Chemistry
  • Analytical Chemistry

Background:

  • Long-range heteronuclear coupling constants are crucial for stereochemical and conformational analysis.
  • Existing methods for measuring these couplings can be time-consuming or lack sensitivity.

Purpose of the Study:

  • To introduce and validate a real-time Zangger-Sterk CPMG-HSQMBC method for direct measurement of multiple-bond heteronuclear couplings.
  • To compare the new method's performance against previously published HSQMBC variants.

Main Methods:

  • Implementation of a real-time acquisition strategy with multiple slice selective excitation.
  • Application of the Zangger-Sterk pulse sequence within a CPMG-HSQMBC framework.
  • Review of decoupling strategies and provision of practical guidelines.

Main Results:

  • The real-time acquisition strategy significantly improves sensitivity or reduces experimental time.
  • The method allows for precise and direct measurement of multiple heteronuclear couplings.
  • Successful applications demonstrated for phosphorus-31 (31P) and selenium-77 (77Se) nuclei.

Conclusions:

  • The developed real-time Zangger-Sterk CPMG-HSQMBC method offers a superior alternative for measuring long-range heteronuclear couplings.
  • This advancement benefits stereochemical and conformational analysis of diverse molecules.
  • The study provides practical insights for selecting appropriate NMR methods.