Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Gas Chromatography: Types of Detectors-II01:19

Gas Chromatography: Types of Detectors-II

1.3K
In gas chromatography, different detectors are employed to meet specific analytical needs. These detectors are often categorized based on their detection mechanisms and the types of compounds they are best suited to analyze. Thermal Conductivity Detectors (TCD), Flame Ionization Detectors (FID), and Electron Capture Detectors (ECD) represent common categories, each with unique operating principles and applications. However, beyond these, several other detectors are designed for more specialized...
1.3K
Emission Spectra02:39

Emission Spectra

76.8K
When solids, liquids, or condensed gases are heated sufficiently, they radiate some of the excess energy as light. Photons produced in this manner have a range of energies, and thereby produce a continuous spectrum in which an unbroken series of wavelengths is present.
76.8K
UV–Vis Spectrometers01:14

UV–Vis Spectrometers

3.8K
The absorbance of UV and visible (UV–visible) radiations is measured using a UV–visible spectrophotometer. Deuterium lamps, which emit UV radiation, and tungsten lamps, which produce radiation in the visible region, are used as light sources in UV–visible spectrophotometers. A monochromator or prism is used for diffraction grating, i.e., to split the incoming radiation into different wavelengths. A system of slits is used to focus the desired wavelength on the sample cell.
3.8K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Electrochemiluminescence microscopy for real-time, single-cell imaging of surface charge in electroactive bacteria.

Nature communications·2026
Same author

Operando Nanocavity-Confined Raman Spectroscopy Uncovers a Drilling-Shearing Mechanism in Nanoplastic Photodegradation.

Journal of the American Chemical Society·2026
Same author

An Entropy-Driven Tetrahedral Framework Nucleic Acid Circuit for Assessing Drug Response during Apoptosis through Intracellular MicroRNA Imaging.

Analytical chemistry·2026
Same author

Nuclear DNA-Gated Electrochemiluminescence Microscopy with Deep Learning for Single-Cell Apoptosis Profiling.

Analytical chemistry·2026
Same author

Precisely Modulating the Interfacial Oxygen Radicals for Selective Photocatalytic Methane Partial Oxidation to Methanol.

Angewandte Chemie (International ed. in English)·2026
Same author

Photoactivatable Cascade DNAzyme Nanomachines for Amplified Imaging of Tumor Response to STING-Activated Immunotherapy.

Analytical chemistry·2026

Related Experiment Video

Updated: Feb 16, 2026

A Sensitive Visual Method for the Detection of Hydrogen Sulfide Producing Bacteria
03:55

A Sensitive Visual Method for the Detection of Hydrogen Sulfide Producing Bacteria

Published on: June 27, 2022

4.2K

A Spectral Shift-Based Electrochemiluminescence Sensor for Hydrogen Sulfide.

Cheng Ma1, Wanwan Wu1, Yujiao Peng1

  • 1State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University , Nanjing 210023, People's Republic of China.

Analytical Chemistry
|December 16, 2017
PubMed
Summary

A novel electrochemiluminescence (ECL) assay uses wavelength shifts instead of intensity changes for accurate detection. This method overcomes interference in complex samples, offering reliable quantitative analysis for various applications.

More Related Videos

Protein Film Infrared Electrochemistry Demonstrated for Study of H2 Oxidation by a [NiFe] Hydrogenase
10:01

Protein Film Infrared Electrochemistry Demonstrated for Study of H2 Oxidation by a [NiFe] Hydrogenase

Published on: December 4, 2017

12.8K
High Spatial Resolution Chemical Imaging of Implant-Associated Infections with X-ray Excited Luminescence Chemical Imaging Through Tissue
07:48

High Spatial Resolution Chemical Imaging of Implant-Associated Infections with X-ray Excited Luminescence Chemical Imaging Through Tissue

Published on: September 30, 2022

1.7K

Related Experiment Videos

Last Updated: Feb 16, 2026

A Sensitive Visual Method for the Detection of Hydrogen Sulfide Producing Bacteria
03:55

A Sensitive Visual Method for the Detection of Hydrogen Sulfide Producing Bacteria

Published on: June 27, 2022

4.2K
Protein Film Infrared Electrochemistry Demonstrated for Study of H2 Oxidation by a [NiFe] Hydrogenase
10:01

Protein Film Infrared Electrochemistry Demonstrated for Study of H2 Oxidation by a [NiFe] Hydrogenase

Published on: December 4, 2017

12.8K
High Spatial Resolution Chemical Imaging of Implant-Associated Infections with X-ray Excited Luminescence Chemical Imaging Through Tissue
07:48

High Spatial Resolution Chemical Imaging of Implant-Associated Infections with X-ray Excited Luminescence Chemical Imaging Through Tissue

Published on: September 30, 2022

1.7K

Area of Science:

  • Analytical Chemistry
  • Materials Science

Background:

  • Traditional electrochemiluminescence (ECL) assays struggle with quantitative analysis in complex samples due to luminescence intensity fluctuations.
  • Existing methods face challenges in maintaining accuracy and reliability when analyzing intricate biological or environmental matrices.

Purpose of the Study:

  • To develop a new sensing strategy for quantitative detection using the maximum luminescence wavelength (λmax) shift as the readout in ECL assays.
  • To overcome the limitations of intensity-based ECL detection by utilizing spectral shifts for improved accuracy.

Main Methods:

  • Designed a novel ECL sensing strategy incorporating an ECL luminophore (RuSiO2@GO) and a hydrogen sulfide (H2S)-sensitive inner filter absorber (CouMC).
  • Utilized the concentration-dependent absorbance change of CouMC to induce a spectral shift (Δλmax) in the ECL luminophore's emission.
  • Employed both experimental validation and computational simulations to confirm the sensing mechanism.

Main Results:

  • Demonstrated that the spectral shift of ECL effectively circumvents interference from total luminescence intensity variations.
  • Achieved highly reliable quantitative analysis by correlating the H2S concentration with the induced wavelength shift.
  • Validated the robustness of the spectral shift-based approach in complex sample analysis.

Conclusions:

  • The developed spectral shift-based ECL assay provides a reliable method for quantitative detection, overcoming common interferences.
  • This innovative strategy broadens the scope of ECL applications by enabling the use of diverse luminophores and absorptive chemodosimeters.
  • The inner filter effect mechanism offers a promising avenue for developing advanced biosensors and chemical sensors.