Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A multiple-encrypted DNA device for secure communication.

Science advances·2026
Same author

Development and validation of a cross-sectional risk screening nomogram for carotid plaque based on routine health examination data and the triglyceride-glucose-waist-to-hip ratio.

Frontiers in endocrinology·2026
Same author

Sensitizing carbapenem-resistant Klebsiella pneumoniae using a compact Cascade-Cas3 system.

International microbiology : the official journal of the Spanish Society for Microbiology·2026
Same author

Phage-mediated expansion of the virulence gene types and enhanced ecological integration of pathogens in wild mice from human-impacted environments.

NPJ biofilms and microbiomes·2026
Same author

Programming Dimensional Transitions in DNA Brick Crystals via Interfacial Connectivity.

Angewandte Chemie (International ed. in English)·2026
Same author

Predicting short-term composite outcome risk in heart failure patients using a machine learning model incorporating UHR: a retrospective cohort study.

Frontiers in nutrition·2026
Same journal

Electrospun Liquid Crystal Elastomers as Stress-Free Thermo- and Photoresponsive Actuators.

ACS applied materials & interfaces·2026
Same journal

Tunable Electrical Transport and Magnetic Anisotropy in Textured SrRuO<sub>3</sub> Films Mediated by Gap Control of Monolayer Ca<sub>2</sub>Nb<sub>3</sub>O<sub>10</sub> Nanosheet Templates.

ACS applied materials & interfaces·2026
Same journal

Label-Free Capacitive Immunosensing of Lactate Dehydrogenase and Interleukin-6 Using a Protein-Passivated Graphene Interface.

ACS applied materials & interfaces·2026
Same journal

Improved Carrier Transport and Enhanced Detection Sensitivity Through Zr<sup>4+</sup> Doping in LiYMo<sub>2</sub>O<sub>8</sub> Single Crystals for X-ray Detectors.

ACS applied materials & interfaces·2026
Same journal

Near-Infrared Light-Driven Microgrooved UCNPs/Azobenzene-LCE Actuators and Substrates for Cardiomyoblast Alignment.

ACS applied materials & interfaces·2026
Same journal

Recent Advances in Superlattice-Based Thermoelectrics.

ACS applied materials & interfaces·2026
See all related articles

Related Experiment Video

Updated: Feb 16, 2026

Phthalic Acid Ester-Binding DNA Aptamer Selection, Characterization, and Application to an Electrochemical Aptasensor
09:33

Phthalic Acid Ester-Binding DNA Aptamer Selection, Characterization, and Application to an Electrochemical Aptasensor

Published on: March 21, 2018

10.4K

Epitope Binning Assay Using an Electron Transfer-Modulated Aptamer Sensor.

Min Li1,2, Xudong Guo3, Hui Li4

  • 1Division of Physical Biology & Bioimaging Center, Shanghai Institute of Applied Physics, Chinese Academy of Sciences , Shanghai 201800, China.

ACS Applied Materials & Interfaces
|December 16, 2017
PubMed
Summary
This summary is machine-generated.

This study introduces a novel method to measure protein-DNA binding affinity using electron transfer kinetics. This technique offers a simpler approach for developing sensitive diagnostic assays, like prostate-specific antigen (PSA) detection.

Keywords:
binding affinityconformation alterationepitope binninginterfacial electron transferpairwise selection

More Related Videos

Aptamer-Based Target Detection Facilitated by a 3-Stage G-Quadruplex Isothermal Exponential Amplification Reaction
03:38

Aptamer-Based Target Detection Facilitated by a 3-Stage G-Quadruplex Isothermal Exponential Amplification Reaction

Published on: October 6, 2022

1.9K
A Flow Cytometry-Based Cell Surface Protein Binding Assay for Assessing Selectivity and Specificity of an Anticancer Aptamer
10:46

A Flow Cytometry-Based Cell Surface Protein Binding Assay for Assessing Selectivity and Specificity of an Anticancer Aptamer

Published on: September 13, 2022

4.4K

Related Experiment Videos

Last Updated: Feb 16, 2026

Phthalic Acid Ester-Binding DNA Aptamer Selection, Characterization, and Application to an Electrochemical Aptasensor
09:33

Phthalic Acid Ester-Binding DNA Aptamer Selection, Characterization, and Application to an Electrochemical Aptasensor

Published on: March 21, 2018

10.4K
Aptamer-Based Target Detection Facilitated by a 3-Stage G-Quadruplex Isothermal Exponential Amplification Reaction
03:38

Aptamer-Based Target Detection Facilitated by a 3-Stage G-Quadruplex Isothermal Exponential Amplification Reaction

Published on: October 6, 2022

1.9K
A Flow Cytometry-Based Cell Surface Protein Binding Assay for Assessing Selectivity and Specificity of an Anticancer Aptamer
10:46

A Flow Cytometry-Based Cell Surface Protein Binding Assay for Assessing Selectivity and Specificity of an Anticancer Aptamer

Published on: September 13, 2022

4.4K

Area of Science:

  • Biochemistry
  • Analytical Chemistry
  • Biosensing

Background:

  • Traditional methods like SPR and QCM for protein-DNA binding analysis are costly and complex.
  • Nonspecific adsorption and technical expertise remain significant barriers.
  • Developing convenient and effective techniques for measuring binding affinity and epitope binning is crucial for sensitive detection platforms.

Purpose of the Study:

  • To develop a novel, convenient, and effective method for measuring protein-DNA binding affinity.
  • To utilize binding-induced alteration in electron transfer kinetics for quantitative analysis.
  • To enable the development of highly sensitive detection platforms, specifically for prostate-specific antigen (PSA).

Main Methods:

  • Developed a method based on electron transfer kinetics of a redox reporter (methylene blue) labeled on a DNA aptamer.
  • Measured the binding affinity between prostate-specific antigen (PSA) and its aptamer.
  • Sorted pairwise aptamer selections from 10 modes to develop a sandwich assay.

Main Results:

  • Demonstrated that PSA binding to the aptamer decreases the electron transfer rate of methylene blue by approximately 45%.
  • Identified optimal aptamer pairs for a sandwich assay.
  • Achieved a PSA detection limit of 500 ng/mL.

Conclusions:

  • The developed method provides a promising approach for analyzing ligand-receptor binding affinity.
  • This technique facilitates the sorting of aptamer or antibody pairs for sensitive sandwich immunoassay development.
  • Offers a simpler and potentially more cost-effective alternative to existing protein-DNA interaction analysis methods.