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Related Concept Videos

Notch Signaling Pathway03:14

Notch Signaling Pathway

6.6K
The Notch signaling pathway is a major intracellular signaling pathway that is highly conserved over a broad spectrum of metazoan species. It stands unique from other intracellular signaling mechanisms in animals because notch protein itself acts as the receptor as well as the primary signaling molecule.
The Notch gene came into the limelight in 1914 after the discovery that its mutation in Drosophila melanogaster leads to a serrated (or "notched") wing margin phenotype. It was not...
6.6K
Role Of Notch Signalling In Intestinal Stem Cell Renewal01:12

Role Of Notch Signalling In Intestinal Stem Cell Renewal

2.5K
Notch signaling was first discovered in Drosophila melanogaster, where it is involved in cell lineage differentiation. Notch signaling regulates the maintenance and differentiation of intestinal stem cells or ISCs by controlling the expression of atonal homolog 1 or Atoh1. Atoh1 directs cells to differentiate into secretory cells.
Direct cell-to-cell contact is needed for the activation of Notch signaling. The signal is initiated when a notch ligand binds to a receptor on an adjacent cell, also...
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What is Cell Signaling?02:03

What is Cell Signaling?

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Despite the protective membrane that separates a cell from the environment, cells need the ability to detect and respond to environmental changes. Additionally, cells often need to communicate with one another. Unicellular and multicellular organisms use a variety of cell signaling mechanisms to communicate to respond to the environment.
131.2K
Overview of Cell Signaling01:23

Overview of Cell Signaling

25.1K
Despite the protective membrane that separates a cell from the environment, cells need the ability to detect and respond to environmental changes. Additionally, cells often need to communicate with one another. Unicellular and multicellular organisms use a variety of cell signaling mechanisms to communicate with the environment.
Cells respond to many types of information, often through receptor proteins positioned on the membrane. For example, skin cells respond to and transmit touch...
25.1K
Diversity in Cell Signaling Responses01:22

Diversity in Cell Signaling Responses

8.0K
The physiological function of a cell and cellular communication are outcomes of a range of extrinsic signals, intracellular signaling pathways, and cellular responses. No two cell types express the same repertoire of signaling components. Receptors are highly selective for their cognate ligands, but once activated, they can alter multiple cellular processes such as DNA transcription, protein synthesis, and metabolic activity. 
Graded and Abrupt Responses
Some signaling systems generate...
8.0K
Contact-dependent Signaling01:19

Contact-dependent Signaling

47.7K
Contact-dependent signaling, as the name suggests, requires that communicating cells be in direct contact with each other. This is achieved either through receptor-ligand interactions or by specialized cytoplasmic channels that allow the flow of small molecules between cells. In animal cells, channels called gap junctions facilitate contact-dependent signaling in certain tissues, whereas, plasmodesmata perform a similar function in plants.
Gap Junctions
In animal cells, gap junctions are formed...
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Related Experiment Video

Updated: Feb 16, 2026

Cell Aggregation Assays to Evaluate the Binding of the Drosophila Notch with Trans-Ligands and its Inhibition by Cis-Ligands
05:48

Cell Aggregation Assays to Evaluate the Binding of the Drosophila Notch with Trans-Ligands and its Inhibition by Cis-Ligands

Published on: January 2, 2018

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Redundancy and cooperation in Notch intercellular signaling.

Juan C Luna-Escalante1, Pau Formosa-Jordan1, Marta Ibañes2,3

  • 1Departament de Física de la Matèria Condensada, Universitat de Barcelona, Barcelona 08028, Spain.

Development (Cambridge, England)
|December 16, 2017
PubMed
Summary

Notch signaling uses multiple ligands to coordinate cell differentiation. Distinct ligand efficiencies create cooperative effects, enhancing signaling robustness and evolvability in development.

Keywords:
Lateral inductionLateral inhibitionMathematical modelingNotch signaling

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Last Updated: Feb 16, 2026

Cell Aggregation Assays to Evaluate the Binding of the Drosophila Notch with Trans-Ligands and its Inhibition by Cis-Ligands
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Area of Science:

  • Developmental Biology
  • Cell Signaling
  • Systems Biology

Background:

  • Notch signaling is crucial for metazoan development, controlling cell differentiation through cell-cell communication.
  • Ligand efficiency and regulation complexity can obscure spatial coordination in Notch pathways.

Purpose of the Study:

  • To investigate how multiple ligands with varying efficiencies impact Notch signaling coordination.
  • To elucidate the cooperative mechanisms and emergent properties in multi-ligand Notch signaling scenarios.

Main Methods:

  • Mathematical modeling of cell-cell communication networks.
  • Computational analysis of ligand-receptor interactions and regulatory feedback.

Main Results:

  • Competing ligands with distinct efficiencies cooperate to generate novel signaling states, increasing robustness and evolvability.
  • Antagonistically regulated ligands enhance the dominant ligand's response.
  • Equivalently regulated ligands lead to emergent spatial patterns dependent on both ligands.

Conclusions:

  • Ligand efficiency is a critical factor in multi-ligand Notch signaling systems.
  • Cooperative ligand interactions provide robustness and evolvability, explaining complex developmental phenotypes.