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Updated: Feb 16, 2026

Metabolic Labeling of Newly Transcribed RNA for High Resolution Gene Expression Profiling of RNA Synthesis, Processing and Decay in Cell Culture
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Metabolic Labeling of Newly Transcribed RNA for High Resolution Gene Expression Profiling of RNA Synthesis, Processing and Decay in Cell Culture

Published on: August 8, 2013

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Determining mRNA half-lives on a transcriptome-wide scale.

Andrew Lugowski1, Beth Nicholson2, Olivia S Rissland1

  • 1Molecular Medicine Program, The Hospital for Sick Children Research Institute, Toronto, ON M5G 0A4, Canada; Department of Molecular Genetics, University of Toronto, Toronto, ON M5S 1A8, Canada.

Methods (San Diego, Calif.)
|December 17, 2017
PubMed
Summary

This study details methods for measuring messenger RNA (mRNA) stability across the entire transcriptome. Understanding mRNA decay is crucial for regulating gene expression and biological processes.

Keywords:
Metabolic labelingTranscription shut-offmRNA decay

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