Related Experiment Video
Updated: Feb 16, 2026

A Protocol for Analyzing Hepatitis C Virus Replication
Published on: June 26, 2014
Coronaviruses and arteriviruses display striking differences in their cyclophilin A-dependence during replication in
Adriaan H de Wilde1, Jessika C Zevenhoven-Dobbe1, Corrine Beugeling1
1Molecular Virology Laboratory, Department of Medical Microbiology, Leiden University Medical Center, Leiden, The Netherlands.
Abstract:
Cyclophilin A (CypA) is an important host factor in the replication of a variety of RNA viruses. Also the replication of several nidoviruses was reported to depend on CypA, although possibly not to the same extent. These prior studies are difficult to compare, since different nidoviruses, cell lines and experimental set-ups were used. Here, we investigated the CypA dependence of three distantly related nidoviruses that can all replicate in Huh7 cells: the arterivirus equine arteritis virus (EAV), the alphacoronavirus human coronavirus 229E (HCoV-229E), and the betacoronavirus Middle East respiratory syndrome coronavirus (MERS-CoV). The replication of these viruses was compared in the same parental Huh7 cells and in CypA-knockout Huh7 cells generated using CRISPR/Cas9-technology. CypA depletion reduced EAV yields by ~ 3-log, whereas MERS-CoV progeny titers were modestly reduced (3-fold) and HCoV-229E replication was unchanged. This study reveals that the replication of nidoviruses can differ strikingly in its dependence on cellular CypA.
Related Concept Videos
Size and Structure of Viral Genomes
Viruses of Archaea
Retrovirus Life Cycles
Intracellular Movement of Viruses and Bacteria
Viral Replication: Lytic Cycle
Viruses with RNA Genomes

