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Laminaria digitata phlorotannins decrease protein degradation and methanogenesis during in vitro ruminal

Anne M Vissers1, Wilbert F Pellikaan2, Anouk Bouwhuis1

  • 1Wageningen University and Research, Laboratory of Food Chemistry, Bornse Weilanden, WG, Wageningen, The Netherlands.

Journal of the Science of Food and Agriculture
|December 19, 2017
PubMed
Summary
This summary is machine-generated.

Marine phlorotannins (PhTs) effectively protect dietary protein from ruminal fermentation and significantly reduce methane production. An optimal dosage of 40 g/kg demonstrated these benefits without negatively impacting overall fermentation.

Keywords:
methanogenesisphlorotanninsprotein degradationruminal fermentation

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Area of Science:

  • Marine natural products chemistry
  • Ruminant nutrition
  • Environmental science

Background:

  • Phlorotannins (PhTs) are marine polyphenols with protein-binding properties.
  • PhTs are hypothesized to protect dietary protein from degradation in the rumen.
  • This study investigates PhTs from Laminaria digitata for their effects on ruminal fermentation.

Purpose of the Study:

  • To evaluate the efficacy of phlorotannins (PhTs) in protecting dietary protein during ruminal fermentation.
  • To determine the impact of PhTs on methane (CH4) production in vitro.
  • To identify the optimal dosage of PhTs for these effects.

Main Methods:

  • In vitro incubation of tannin-free grass silage with varying concentrations of a methanolic PhTs extract (10-100 g/kg).
  • Assessment of ruminal fluid parameters including gas production, methane (CH4) output, ammonia concentration, and volatile fatty acids.
  • Investigation of polyethylene glycol (PEG) effects to confirm tannin activity.

Main Results:

  • Phlorotannins (PhTs) exhibited significant linear and quadratic effects on gas and methane production.
  • An optimal PhTs dosage of 40 g/kg reduced methane (CH4) from 24.5 to 15.2 mL/g OM without altering gas or volatile fatty acid production.
  • Ammonia levels showed a decreasing trend, suggesting protein protection, while PEG addition negated PhTs' effects.

Conclusions:

  • Phlorotannins (PhTs) effectively protect dietary protein from ruminal degradation.
  • PhTs significantly reduce ruminal methanogenesis in a dose-dependent manner.
  • The optimal dosage for PhTs was determined to be 40 g/kg for mitigating methane emissions.