NDRG4 promotes myogenesis via Akt/CREB activation
Mingfei Zhu1, Rong Zheng1, Yiwen Guo1
1Key Laboratory of Swine Genetics and Breeding of Ministry of Agriculture & Key Laboratory of Agriculture Animal Genetics, Breeding and Reproduction of Ministry of Education, College of Animal Science and Technology, Huazhong Agricultural University, Wuhan 430070, P.R. China.
Oncotarget
|December 20, 2017
Summary
N-Myc downstream-regulated gene 4 (NDRG4) significantly enhances muscle development by promoting myogenic differentiation. It activates the Akt/CREB pathway, boosting key muscle gene expression and myotube formation.
Area of Science:
- Molecular Biology
- Cell Biology
- Muscle Physiology
Background:
- N-Myc downstream-regulated gene 4 (NDRG4) is implicated in various biological processes and diseases.
- The specific role of NDRG4 in muscle development and myogenic differentiation remains largely unexplored.
Purpose of the Study:
- To elucidate the function of NDRG4 in regulating myogenic differentiation.
- To investigate the molecular mechanisms underlying NDRG4's role in muscle development.
Main Methods:
- Analysis of NDRG4 expression during muscle regeneration and C2C12 myoblast differentiation.
- Gain-of-function and loss-of-function studies to assess NDRG4's impact on myogenic markers.
- Investigation of the interaction between NDRG4, CTMP, Akt, and CREB signaling pathways.
Main Results:
- NDRG4 expression increases during muscle regeneration and C2C12 cell differentiation.
- NDRG4 overexpression promotes myogenic differentiation, increasing MyoD, MyoG, and MyHC gene expression and myotube formation.
- NDRG4 binds to CTMP, disrupting CTMP-Akt interaction, leading to increased Akt and CREB phosphorylation.
Conclusions:
- NDRG4 is a positive regulator of myogenic differentiation.
- NDRG4 promotes muscle development by activating the Akt/CREB signaling pathway.
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