Simple gamete preservation and artificial reproduction of mammals using micro-insemination techniques

Assisted reproductive technology (ART) has been applied in various procedures as an effective breeding method in experimental, domestic, and wild animals, and for the treatment of human infertility. Micro-insemination techniques such as intracytoplasmic injection of spermatozoa and spermatids are now routinely used ART tools. With these techniques, even immotile and immature sperm cells can be employed as donors for producing the next generation. Gamete preservation, another ART tool, has contributed to reproductive regulation, worldwide transportation, and disease protection of animal strains, and the preserved gametes have been effectively used for the production of offspring. ART is now an indispensable tool in mammalian reproduction. This review covers the latest ART tools, with a particular emphasis on micro-insemination and gamete preservation, and discusses the future direction of mammalian artificial reproductive technology.