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Human LYPD8 protein inhibits motility of flagellated bacteria

Chiao-Ching Hsu1,2,3, Ryu Okumura1,2,3, Kiyoshi Takeda1,2,3

  • 1Department of Microbiology and Immunology, Graduate School of Medicine, Osaka University, Osaka, 565-0871 Japan.

Inflammation and Regeneration
|December 21, 2017
PubMed
Abstract

Insights

Human LYPD8 (hLYPD8) protein inhibits flagellated bacteria motility, potentially preventing intestinal inflammation. This finding offers a new therapeutic strategy for inflammatory bowel diseases by targeting bacterial activity.

Area of Science:

  • Gastroenterology and Immunology
  • Microbiology
  • Protein Biochemistry

Background:

  • Mouse Ly6/Plaur domain containing 8 (mLypd8) prevents intestinal inflammation by segregating microbiota.
  • Human LYPD8 (hLYPD8) is expressed in colonic epithelia, with reduced levels in ulcerative colitis patients.
  • The molecular characteristics and functions of hLYPD8 are not well understood.

Purpose of the Study:

  • To generate and characterize the human LYPD8 (hLYPD8) protein.
  • To investigate the functional properties of hLYPD8, particularly its interaction with flagellated bacteria.

Main Methods:

  • Recombinant FLAG-tagged hLYPD8 protein produced using mammalian and Pichia pastoris expression systems.
  • Western blot, deglycosylation assays, ELISA, and bacterial motility assays were employed for characterization.

Main Results:

  • hLYPD8 is a highly N-glycosylated, GPI-anchored protein, similar to mLypd8.
  • Recombinant hLYPD8 from Pichia pastoris exhibited high binding affinity and motility inhibition against flagellated bacteria like Proteus mirabilis.
  • The Pichia pastoris SuperMan5 strain facilitated production of hLYPD8 with human-like glycosylation.

Conclusions:

  • hLYPD8 effectively inhibits the motility of flagellated bacteria, many implicated in intestinal inflammation.
  • Supplementation with recombinant hLYPD8 protein presents a potential novel therapeutic approach for inflammatory bowel diseases.
Keywords:
Flagellated bacteriaGlycosylationIntestinal inflammationLYPD8Pichia Pastoris

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