Ultrastructural evidence for the identity of some multinucleate rhizoctonias

The ultrastructure of the pore cap in ten multinucleate rhizoctonias, endophytes of non-orchids and of Microtis (Orchidaceae), was compared with that in isolates of four Sebacina species. The pore cap in all the rhizoctonias was an imperforate, disc-like structure, Slightly smaller in diameter than the flange forming the septal pore canal. The cap was formed by two Bat lamellae continuous with, but more electron-dense than, the membranes of the endoplasmic reticulum. The lamellae enclosed a lumen containing a deposit of electron-dense material. The pore cap in the rhizoctonias was indistinguishable from that in an isolate of Sebacina vermifera Oberwinkler. In contrast, the pore caps in isolates of S. calcea (Pers.: Fr.) Bres., S. umbrina Rogers and S. grisea (Pets.: Fr.) Bres., which were also imperforate and contained a band of electron dense material, were strongly dome-shaped and extended from one side of the flange to the other.