Related Experiment Video
Updated: Feb 16, 2026
A Precision Medicine Tool for Measurement and Monitoring of Hemoglobin S in Sickle Cell Disease Patients Receiving Transfusion Therapy
Comparison of HbA2, E, F and Red Cell Parameters in Homozygous HbE With and Without α0-Thalassemia Trait
Sakorn Pornprasert1, Somkhid Thichak2, Kanyakan Kongthai3
1Department of Medical Technology, Faculty of Associated Medical Sciences, Chiang Mai University, Chiang Mai, Thailand.
Objectives:
To compare levels of HbA2, HbE, HbF, and red cell parameters (total Hb, PCV, MCV, and MCH) and also determine their appropriated cut-off points for initial discrimination between homozygous HbE with and without α0-thalassemia trait.
Methods:
Hb analysis results from capillary electrophoresis (CE) and red cell parameters of homozygous HbE without α0-thalassemia trait (n = 41) and with α0-thalassemia trait (n = 17) were reviewed.
Results:
The MCV, MCH, and HbE of homozygous HbE with α0-thalassemia trait were significantly lower than those of homozygous HbE without α0-thalassemia, while HbA2 levels of the former were significantly higher than those of the latter. HbA2 at a cut-off point of 5.3% had 69.0% efficiency in discrimination between the 2 groups. It could also reduce 56.1% of homozygous HbE samples for α0-thalassemia testing.
Conclusions:
The elevated HbA2 ≥5.3% is a useful marker for initial discrimination between homozygous HbE with and without α0-thalassemia trait.
More Related Videos
11:59Detection of Residual Donor Erythroid Progenitor Cells after Hematopoietic Stem Cell Transplantation for Patients with Hemoglobinopathies
Published on: September 6, 2017
08:23Characterization of Sickling During Controlled Automated Deoxygenation with Oxygen Gradient Ektacytometry
Published on: November 5, 2019
Related Concept Videos
01:49Multiple Allele Traits
02:20Blood Types
ABO blood group
ABO antigens are glycoproteins encoded by genes present on...
01:24Hemoglobin
When all four heme groups are bound to oxygen, the resulting molecule is called oxyhemoglobin. As a result, arterial blood...
01:10Blood Typing
Antigens are protein molecules that reside on the surface of red blood cells (RBCs). The ABO and Rh blood typing systems target...
01:12The ABO Blood Group
Antigens in the ABO Blood Group System
Antigens are substances that can trigger an immune response, leading to the production of antibodies. In the ABO blood group system,...
02:45Blood Transfusion and Agglutination
History
The history of blood transfusion dates back to the 17th century, when early attempts were made in animals. In 1818 James Blundell, a British doctor, performed the first successful human blood transfusion. Later in 1900, Karl...