A novel fluorescein-based "turn-on" probe for the detection of hydrazine and its application in living cells
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Application of Genetically Encoded Fluorescent Nitric Oxide (NO•) Probes, the geNOps, for Real-time Imaging of NO• Signals in Single Cells
Published on: March 16, 2017
Wen-Zhi Xu1, Wei-Yan Liu1, Ting-Ting Zhou1
1Key Laboratory of Medicinal Chemistry and Molecular Diagnosis of the Ministry of Education, College of Chemistry & Environmental Science, Hebei University, Baoding 071002, PR China.
Abstract:
We constructed a novel probe for hydrazine detection based on ICT and PET mechanism. Phthalimide and acetyl ester groups were used as the recognition units. Addition of hydrazine produced a turn-on fluorescence at 525nm along with the fluorescent color change from dark to yellow. The probe could selectively detect hydrazine over other related interfering species. The detection limit of the probe for hydrazine was calculated to be 0.057μM which was lower than the EPA standard (0.320μM). Furthermore, the probe could also be applied for the imaging of hydrazine in living cells.
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