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Tangential Flow Ultrafiltration: A “Green” Method for the Size Selection and Concentration of Colloidal Silver Nanoparticles
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Shape-Dependent Dissolution and Cellular Uptake of Silver Nanoparticles.

Christina Graf1, Daniel Nordmeyer1, Christina Sengstock2

  • 1Physikalische und Theoretische Chemie, Institut für Chemie und Biochemie, Freie Universität Berlin , 14195 Berlin, Germany.

Langmuir : the ACS Journal of Surfaces and Colloids
|December 24, 2017
PubMed
Summary

Silver nanoprisms and nanospheres showed significant dissolution within 24 hours in human cells. Human mesenchymal stem cells took up more nanoprisms, unlike keratinocytes, due to membrane flexibility differences.

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Area of Science:

  • Nanotechnology
  • Biomaterials Science
  • Cell Biology

Background:

  • Silver nanoparticles (AgNPs) are increasingly used in biomedical applications.
  • Understanding their interaction with cells, including uptake and dissolution, is crucial for safety and efficacy.
  • The shape and surface chemistry of AgNPs influence their biological behavior.

Purpose of the Study:

  • To investigate the cellular uptake and dissolution of silver nanoprisms and nanospheres in human mesenchymal stem cells (hMSCs) and human keratinocytes (HaCaT cells).
  • To correlate particle dissolution rates with cellular uptake mechanisms.
  • To elucidate the role of cell membrane properties in particle-cell interactions.

Main Methods:

  • Synthesis and characterization of trigonal silver nanoprisms and spherical silver nanoparticles.
  • Incubation of cells with silver nanoparticles under controlled conditions.
  • Quantification of silver uptake using elemental analysis.
  • Assessment of particle dissolution in vitro and within cells.
  • Analysis of cell membrane properties (Young's modulus, flexibility).

Main Results:

  • Silver nanoprisms and nanospheres exhibited significant dissolution (>90% volume loss) within 24 hours in both cell types.
  • Nanoprisms dissolved rapidly in saline and at pH 4, with surface passivation by PVP and citrate influencing the rate.
  • hMSCs showed significantly higher uptake of nanoprisms compared to nanospheres.
  • HaCaT cells exhibited no shape preference for silver nanoparticle uptake.
  • Cellular uptake differences were attributed to the interplay between particle shape, cell membrane flexibility, and surface energy.

Conclusions:

  • Silver nanoprisms and nanospheres undergo substantial dissolution in human cells within 24 hours.
  • Cellular uptake of silver nanoparticles is shape-dependent and influenced by the mechanical properties of the cell membrane.
  • hMSC's flexible membranes facilitate greater uptake of platelet-like nanoprisms, while the stiffer membranes of HaCaT cells lead to no shape preference.