Related Experiment Video
Updated: Feb 16, 2026

Combining Behavioral Endocrinology and Experimental Economics: Testosterone and Social Decision Making
Published on: March 2, 2011
Dermal absorption of testosterone in human and pig skin in vitro
Jon R Heylings1, Diane J Davies1, Rebecca Burton1
1Dermal Technology Laboratory Ltd, Med IC4, Keele University Science and Innovation Park, Keele, Staffordshire, United Kingdom.
Abstract:
The OECD test guideline 428 for the assessment of dermal absorption in vitro has been in force for more than a decade. Various sectors of industry utilise the method for the registration of chemical products. These include the Agrochemical and Cosmetic sectors where the OECD test guideline and industry-specific guidance forms a key part of the human risk assessment process for new and existing products. This investigation has compared the dermal absorption characteristics of one of the OECD 428 reference chemicals, testosterone, in human and pig dermatomed skin. We used identical dosing and skin decontamination conditions for testosterone in Franz static diffusion cells. This included a full mass balance recovery of the dose applied and distribution of the compound in the different layers of the skin. Our investigation has shown that intact human skin provides a more effective barrier to the dermal absorption of testosterone compared with pig skin, when studied according to modern day in vitro dermal absorption guidance.
More Related Videos
09:14Isolation of Papillary and Reticular Fibroblasts from Human Skin by Fluorescence-activated Cell Sorting
Published on: May 7, 2019
10:52Isolation of Adult Human Dermal Fibroblasts from Abdominal Skin and Generation of Induced Pluripotent Stem Cells Using a Non-Integrating Method
Published on: January 19, 2020
Related Concept Videos
Testosterone: Functions and Regulation
Methods for Studying Drug Absorption: In vitro
The diffusion cell method uses a two-compartment cell, including a donor compartment with the drug solution, which simulates the environment where the drug is applied, and a receptor compartment with a buffer solution, which simulates the environment...
Drug Absorption: Factors Affecting GI Absorption
Absorption of Radiation
Protein Absorption
Lipid Absorption
These breakdown products bind with bile salts and lecithin to form micelles, which quickly pass between microvilli to come in close contact with the apical...