[Forensic Investigation in Han Population by SiFa™ 23 Plex Kit （Beta Version）]

Area of Science:

• Forensic Science
• Population Genetics
• Molecular Biology

Background:

• Short tandem repeat (STR) loci are crucial for human identification.
• Accurate and efficient genotyping kits are essential for forensic applications.

Purpose of the Study:

• Evaluate the performance of the SiFa™ 23 Plex Kit (beta version).
• Analyze the population genetics of STR loci in the Han population.

Main Methods:

• Genotyping of 1,000 Han individuals using the SiFa™ 23 Plex Kit.
• Statistical analysis of STR loci frequency data and population genetics parameters.
• Assessment of kit efficiency, including minimum amplification volume and DNA input requirements.

Main Results:

• The kit achieved satisfactory genotyping profiles with as little as 125 pg DNA.
• 267 alleles were detected across 21 autosomal STR loci, conforming to Hardy-Weinberg equilibrium.
• Newly added loci (D1S1656, D10S1248) exhibited high polymorphism information content.

Conclusions:

• The SiFa™ 23 Plex Kit (beta version) is highly effective for forensic DNA testing of blood samples.
• The kit's accuracy, repeatability, and sensitivity meet forensic practice requirements.
• The kit is suitable for forensic casework and the establishment of DNA databases.