Related Experiment Video
Updated: Feb 16, 2026
07:48
Myocardial Infarction in Neonatal Mice, A Model of Cardiac Regeneration
Published on: May 24, 2016
19.9K
Direct In Vivo Reprogramming with Sendai Virus Vectors Improves Cardiac Function after Myocardial Infarction.
Kazutaka Miyamoto1, Mizuha Akiyama1, Fumiya Tamura1
1Department of Cardiology, Keio University School of Medicine, 35 Shinanomachi, Shinjuku-ku, Tokyo 160-8582, Japan.
Cell Stem Cell
|December 26, 2017
Summary
Sendai virus vectors efficiently reprogram fibroblasts into cardiomyocyte-like cells (iCMs) without integrating into the genome. This non-integrating method enhances cardiac regeneration and improves heart function after myocardial infarction.
Area of Science:
- Regenerative Medicine
- Cardiovascular Biology
- Gene Therapy
Background:
- Direct cardiac reprogramming offers a promising avenue for regenerative medicine.
- Previous methods using retroviral vectors for Gata4, Mef2c, and Tbx5 (GMT) overexpression resulted in integration-associated risks and inefficiencies.
- Developing safer and more efficient reprogramming tools is crucial for clinical translation.
Purpose of the Study:
- To evaluate the efficacy of Sendai virus (SeV) vectors for direct cardiac reprogramming.
- To compare SeV-mediated reprogramming with traditional retroviral methods.
- To assess the therapeutic potential of SeV-generated induced cardiomyocyte-like cells (iCMs) in a myocardial infarction model.
Main Methods:
- Fibroblasts (mouse and human) were reprogrammed into iCMs using SeV vectors expressing GMT (SeV-GMT).
- Efficiency and kinetics of reprogramming were compared between SeV-GMT and retroviral-GMT.
- In vivo lineage tracing was performed in mouse infarct hearts.
- Cardiac function and fibrosis were assessed after myocardial infarction.
Main Results:
- SeV-GMT achieved significantly higher efficiency (100-fold increase) in generating beating iCMs compared to retroviral-GMT.
- The reprogramming duration was reduced from 30 to 10 days in mouse fibroblasts.
- SeV-GMT demonstrated superior in vivo reprogramming of cardiac fibroblasts and improved cardiac function post-myocardial infarction.
Conclusions:
- Efficient, non-integrating SeV vectors represent a powerful platform for cardiac regeneration.
- SeV-mediated direct cardiac reprogramming offers a safer and more rapid alternative to retroviral methods.
- This approach holds significant potential for treating heart diseases like myocardial infarction.
Related Concept Videos
01:10Direction of Acceleration Vectors
22.9K
Acceleration occurs when velocity changes in magnitude (an increase or decrease in speed), direction, or both. Although acceleration is in the direction of the change in velocity, it is not always in the direction of motion. When an object slows down, its acceleration is opposite to the direction of its motion. This is commonly referred to as deceleration. However, the term deceleration can cause confusion in analysis because it is not a vector; it does not point to a specific direction with...
22.9K
01:29Direction Cosines of a Vector
1.6K
Direction cosines, which help describe the orientation of a vector with respect to the coordinate axes, are an essential concept in the field of vector calculus. Consider vector A that is expressed in terms of the Cartesian vector form using i, j, and k unit vectors. The magnitude of vector A is defined as the square root of the sum of the squares of its components. The direction of this vector with respect to the x, y, and z axes is defined by the coordinate direction angles α, β, and γ,...
1.6K
00:50What are Viruses?
128.5K
Overview
128.5K
01:30Vectors
543
Vectors are mathematical entities characterized by both magnitude and direction. Unlike scalars, which are defined solely by magnitude, vectors represent quantities like displacement, velocity, and force, where direction is essential. Vectors are graphically represented as directed line segments, extending from an initial point to a terminal point, denoted with bold letters or arrows placed above the symbol. Two vectors are deemed equal if they share identical magnitudes and directions,...
543
01:14Introduction to Nuclear Reprogramming
2.3K
Nuclear reprogramming is the process of switching gene expression of one cell type to that of another cell type, usually from a differentiated cell state to an undifferentiated cell state. Differentiation occurs during processes such as development and morphogenesis, tissue regeneration, and malignancy. Cells can also be artificially induced to reprogram their gene expression by techniques such as nuclear transfer, induced pluripotency, and cell fusion. Such techniques have many applications in...
2.3K
01:24Methods of Nuclear Reprogramming
2.2K
Nuclear reprogramming is a process of transforming one cell type into an unrelated cell type by epigenetic changes that alter the cell’s original gene expression pattern. Such epigenetic changes force cells to express a different set of genes, which play a significant role in inducing transformation into other cell types. Nuclear reprogramming offers applications in reproductive cloning for livestock propagation and regenerative medicine — developing patient-specific cells for...
2.2K