Related Experiment Videos
Versatile supplement device with remote control for the control of patch clamp experiments.
V Rohlicek1, U Fröbe, H Gögelein
1Physiological Institute CSAV, Prague, Czechoslovakia.
Pflugers Archiv : European Journal of Physiology
|February 1, 1989
Summary
A novel electronic device enhances patch-clamp electrophysiology. It features an acoustic seal indicator, pulse generators, and remote control for streamlined experiments with patch-clamp amplifiers.
Area of Science:
- Electrophysiology
- Neuroscience
- Biophysics
Background:
- Patch-clamp electrophysiology is crucial for studying ion channel function.
- Optimizing seal formation and experimental control is vital for data quality.
- Existing patch-clamp setups can be complex to operate and integrate.
Purpose of the Study:
- To describe a versatile electronic device for patch-clamp amplifiers.
- To improve the efficiency and ease of patch-clamp experiments.
- To provide enhanced control and monitoring capabilities.
Main Methods:
- Development of an integrated electronic device for patch-clamp amplifiers.
- Inclusion of an acoustic indicator for seal formation monitoring (search-mode).
- Integration of pulse generators, a staircase generator, a low-pass filter, and a remote control unit.
Main Results:
- The device facilitates seal formation detection via an acoustic indicator.
- Pulse and voltage step programs can be generated for various experimental paradigms.
- Remote control allows seamless switching between modes (search, current clamp, voltage clamp) and stimulus program activation.
Conclusions:
- This electronic device offers a versatile and integrated solution for patch-clamp experiments.
- It simplifies experimental control and enhances data acquisition quality.
- The device is easily connectable to existing patch-clamp amplifiers, offering broad applicability.