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Liquid-cell Transmission Electron Microscopy for Tracking Self-assembly of Nanoparticles
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Biocatalytic Self-Assembly on Magnetic Nanoparticles.

Maria P Conte1, Jugal Kishore Sahoo1,2, Yousef M Abul-Haija1,3

  • 1WestCHEM, Department of Pure & Applied Chemistry, University of Strathclyde , 99 George Street, Glasgow G1 1RD, U.K.

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|December 29, 2017
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Summary

Enzymes immobilized on magnetic nanoparticles (NPs) spatially control peptide self-assembly into nanofibers. This NP-enzyme system enhances hydrogel strength and stability, allowing external magnetic field control for nanomaterial fabrication and biomaterials.

Keywords:
biocatalysisenzyme immobilizationenzymesmagnetic nanoparticlespeptidesself-assembly

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Area of Science:

  • Biocatalysis and Nanomaterials Science
  • Biomaterials Engineering
  • Nanofabrication

Background:

  • Catalysis and molecular self-assembly are key for creating ordered nanostructures.
  • Confining catalysts spatially controls self-assembly, inspired by biological cells.
  • Previous work explored catalytic self-assembly for material production.

Purpose of the Study:

  • To use enzymes immobilized on magnetic nanoparticles (NPs) to initiate and control peptide self-assembly.
  • To investigate the impact of NP-localized catalysis on hydrogel formation and properties.
  • To explore external control over self-assembled hydrogel formation and structure.

Main Methods:

  • Immobilizing enzymes onto magnetic nanoparticles (NPs).
  • Initiating peptide self-assembly around enzyme-NP conjugates.
  • Characterizing hydrogel morphology, shear strength, and stability.
  • Applying external magnetic fields to control hydrogel formation and structure.

Main Results:

  • Peptide self-assembly was spatially localized around enzyme-immobilized NPs, forming nanofibers.
  • Hydrogels exhibited a "hub-and-spoke" morphology with enhanced shear strength and stability.
  • The system demonstrated external control over hydrogel formation and structure using magnetic fields.
  • Both equilibrium and nonequilibrium catalytic systems showed improved properties.

Conclusions:

  • Enzyme-immobilized magnetic NPs provide spatial control over peptide self-assembly and hydrogel formation.
  • The NP-enzyme system significantly enhances hydrogel mechanical properties and stability.
  • Magnetic NPs enable external stimuli-responsive control of self-assembled nanomaterials.
  • This approach holds promise for applications in nanomaterial fabrication, biomaterials, and biosensing.