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Updated: Feb 16, 2026

Characterizing Cellular Proteins with In-cell Fast Photochemical Oxidation of Proteins

Characterizing Cellular Proteins with In-cell Fast Photochemical Oxidation of Proteins

Published on: March 11, 2020

Oxidation of proteins: is it a programmed process?

Mark A Rosenfeld¹, Alexandra D Vasilyeva¹, Lyubov V Yurina¹

¹a N. M. Emanuel Institute of Biochemical Physics, Russian Academy of Sciences , Moscow , Russia.

Free Radical Research

|December 30, 2017

Summary

This summary is machine-generated.

Proteins can be damaged by oxidants, but some protein structures may be adapted to scavenge reactive oxygen species (ROS). This review explores programmed protein oxidation mechanisms, particularly in plasma proteins.

Keywords:

Antioxidant defence mechanism fibrin-stabilising factor fibrinogen human serum albumin α2-macroglobulin

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Area of Science:

Biochemistry
Molecular Biology
Oxidative Stress Research

Background:

Proteins are vulnerable to oxidative damage, impacting their structure and function.
Cellular antioxidant defense relies heavily on enzymatic systems.
Plasma proteins have limited enzymatic protection against reactive oxygen species (ROS).

Purpose of the Study:

To investigate the mechanisms of protein oxidation, especially in plasma proteins.
To present evidence for hypothetical antioxidant structures within proteins.
To highlight the concept of programmed protein oxidation.

Main Methods:

Review of existing literature on protein oxidative modifications.
Analysis of oxidative damage in specific plasma proteins (α2-macroglobulin, albumin, fibrinogen, fibrin-stabilising factor).
Examination of protein structural adaptations to oxidation.

Main Results:

Oxidative modification can disrupt protein structure and function.
Evidence suggests site-specific oxidation, implying structural adaptation.
Hypothesized that certain protein structural elements act as ROS scavengers.

Conclusions:

Protein structures may possess inherent antioxidant capabilities.
These 'antioxidant structures' can protect crucial amino acid residues from ROS.
Protein oxidation may be a programmed process rather than random.