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Related Concept Videos

EDTA: Auxiliary Complexing Reagents01:26

EDTA: Auxiliary Complexing Reagents

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EDTA titrations are usually carried out in highly basic conditions, where the fully deprotonated form of EDTA, Y4−, actively complexes with the free metal ions in the solution. Several metal ions precipitate as hydrous oxide (hydroxides, oxides, or oxyhydroxides) under these conditions, lowering the concentration of free metal ions in the solution. For this reason, auxiliary complexing agents or ligands such as ammonia, tartrate, citrate, or triethanolamine are used in EDTA titrations to...
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Acid Halides to Ketones: Gilman Reagent01:14

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Lithium dialkyl cuprate, also known as Gilman reagents, selectively reduces acid halides to ketones. The acid chloride is treated with Gilman reagent at −78 °C in the presence of ether solution to produce a ketone in good yield.
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Preparation of Carboxylic Acids: Carboxylation of Grignard Reagents01:13

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Carboxylic acids can be prepared by the carboxylation of Grignard reagents (RMgX). This method is convenient for converting alkyl (primary, secondary or tertiary), vinyl, benzyl, and aryl halides to carboxylic acids with one additional carbon than the starting RMgX.
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Field Effect Transistor01:29

Field Effect Transistor

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Field-effect transistors (FETs) are integral to electronic circuits and distinguished by their three-terminal setup: the gate, drain, and source. These transistors operate as unipolar devices, which utilize either electrons or holes as charge carriers, in contrast to bipolar transistors, which use both types of carriers. The primary function of the FET is to modulate the flow of these carriers from the source to the drain through a channel. The voltage difference between the gate and source...
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Electric Field01:16

Electric Field

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Consider two point charges, each exerting Coulomb force on the other. It is possible to describe the Coulomb interaction via an intermediate step by defining a new physical quantity called the electric field.
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Magnetic Fields01:27

Magnetic Fields

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A moving charge or a current creates a magnetic field in the surrounding space, in addition to its electric field. The magnetic field exerts a force on any other moving charge or current that is present in the field. Like an electric field, the magnetic field is also a vector field. At any position, the direction of the magnetic field is defined as the direction in which the north pole of a compass needle points.
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Rapid Diagnosis of Avian Influenza Virus in Wild Birds: Use of a Portable rRT-PCR and Freeze-dried Reagents in the Field
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A Field-Portable Cell Analyzer without a Microscope and Reagents.

Dongmin Seo1, Sangwoo Oh2,3, Moonjin Lee4

  • 1Department of Electronics and Information Engineering, Korea University, Sejong 30019, Korea. ehdals20907@korea.ac.kr.

Sensors (Basel, Switzerland)
|December 30, 2017
PubMed
Summary
This summary is machine-generated.

This study introduces NaviCell, a portable, reagent-free cell analyzer. It accurately measures cell count and viability using lens-free imaging, outperforming traditional methods.

Keywords:
Lens-Free Shadow Imaging Technology (LSIT)automatic cell analysis algorithmcell countinglens-free cell analyzerreagent-freeviability analysis

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Area of Science:

  • Biomedical Engineering
  • Cell Biology
  • Analytical Chemistry

Background:

  • Accurate cell counting and viability assessment are crucial for biological research and clinical diagnostics.
  • Traditional methods often require microscopy, staining reagents, and manual sample preparation, which can be time-consuming and prone to errors.
  • There is a need for automated, portable, and reagent-free solutions for cell analysis.

Purpose of the Study:

  • To demonstrate a commercial-level, field-portable lens-free cell analyzer, NaviCell (No-stain and Automated Versatile Innovative cell analyzer).
  • To evaluate the performance of NaviCell for automated cell counting and viability testing without reagents.
  • To compare NaviCell's accuracy and precision against the hemocytometer and a commercial cell counter.

Main Methods:

  • Utilized a lens-free shadow imaging technique for cell analysis.
  • Developed a compact and portable device (162 × 135 × 138 mm³, 1.02 kg) with a large field of view.
  • Tested performance using 13 cell lines for counting and six cell lines for viability, comparing results against hemocytometer standards.

Main Results:

  • NaviCell achieved significantly improved performance metrics compared to a commercial cell counter.
  • Error rate (ER) and coefficient of variation (CV) for cell counting were 3.27 and 2.16 times better, respectively.
  • ER and CV for cell viability testing were 5.09 and 1.8 times better, respectively, demonstrating high accuracy and reproducibility.

Conclusions:

  • NaviCell offers a robust, reagent-free, and automated solution for cell count and viability analysis.
  • The lens-free imaging technology provides a simplified workflow with reduced potential for sample preparation errors.
  • NaviCell demonstrates substantial potential for diverse applications in cell analysis, research, and diagnostics.