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Updated: Feb 16, 2026

In Vitro Growth of Mouse Preantral Follicles Under Simulated Microgravity
Published on: December 17, 2017
In Vitro Growth of Mouse Preantral Follicles Under Simulated Microgravity
Shen Zhang1, Yonggen Wu1, Yimin Weng2
1Reproductive Medicine Center, The First Affiliated Hospital of Wenzhou Medical University.
Abstract:
14 day-old mouse ovarian tissue and preantral follicles isolated from same-aged mice were incubated in a simulated microgravity culture system. We quantitatively assessed follicle survival, measured follicle and oocyte diameters, and examined ultrastructure of the oocytes produced from the system. We observed decreased follicle survival, downregulation of expressions of proliferating cell nuclear antigen and growth differentiation factor 9, as indicators for the development of granulosa cells and oocytes, respectively, and oocyte ultrastructural abnormalities under the simulated microgravity condition. The simulated microgravity experimental setup needs to be optimized to provide a model for investigation of the mechanisms involved in the oocyte/follicle in vitro development.
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